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Application of total alkaloids of Jingdongshan orange in the preparation of drugs for preventing and treating inflammatory bowel disease

A technology for inflammatory bowel disease and total alkaloids, applied in the field of medicine, can solve the problems of prevention and treatment of inflammatory bowel disease, such as no patent literature reports, etc., meet market demand and promising prospects, huge social and economic effects, and convenient collection Effect

Active Publication Date: 2021-06-22
云南聚和生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jingdongshan orange is rich in indole alkaloids, terpenoids, sterols and volatile oils, which have a good regulatory effect on the physiological functions of the body; Enteropathy research has no patent literature report

Method used

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  • Application of total alkaloids of Jingdongshan orange in the preparation of drugs for preventing and treating inflammatory bowel disease
  • Application of total alkaloids of Jingdongshan orange in the preparation of drugs for preventing and treating inflammatory bowel disease
  • Application of total alkaloids of Jingdongshan orange in the preparation of drugs for preventing and treating inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Extraction and Preparation of Jingdongshan Orange Total Alkaloids

[0023] Take by weighing 100g of pulverized Jingdongshan orange branches and leaves, according to the ratio of solid-liquid ratio g:mL=1:10, use methanol aqueous solution with a volume concentration of 90% for ultrasonic extraction for 30min, filter, and after repeated ultrasonic extraction of filter residue for 3 times, combine the filtrates, The extract was obtained after evaporation and concentration at 45°C; the extract was dissolved in aqueous hydrochloric acid solution with a volume concentration of 0.3%, adjusted to pH 2-3, extracted with ethyl acetate, the water layer was collected, and then ammonia water with a volume concentration of 5% was added. Adjust the pH to 9-10, then extract with ethyl acetate, collect the ethyl acetate layer, evaporate and concentrate, freeze-dry to obtain the total alkaloids of Jingdongshan orange.

Embodiment 2

[0024] Embodiment 2: Apply the method of DSS induction to establish the model of UC, observe the preventive effect of total alkaloids of Jingdongshan orange on UC through animal model experiments

[0025] 1. Establishment of UC model and experimental process

[0026] Male C57BL / 6 mice (6-8 weeks, weight 21-23g) were used as research objects, with 8 mice in each group; the extreme concentration was established by administration of 2.5% DSS (dextran sodium sulfate) for 7 consecutive days 5-aminosalicylic acid (SASP) was used as a positive drug, and the total alkaloids of Jingdongshan orange were administered to evaluate its preventive effect on UC; the mice were adapted to the standard environment for seven days after purchase, and the standard Feed for feeding. The specific experimental procedures and groups are as follows:

[0027] Negative control group (Control): normal drinking water

[0028] Model group (Model): administrated with normal saline for 10 consecutive days, ...

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Abstract

The invention discloses a new application of the total alkaloids of Jingdongshan orange, that is, the application in the preparation of drugs for the prevention and / or treatment of inflammatory bowel disease, and belongs to the field of natural medicine development or medical technology; the invention uses the aerial parts of Jingdongshan orange As a raw material, the total alkaloids of Jingdongshan orange are obtained by ultrasonic extraction with methanol water, and the total alkaloids of Jingdongshan orange are obtained through acid-base treatment; Jingdongshan orange plants are widely distributed, the price is low, the raw materials are easy to obtain, and the alkaloid extraction process is simple; the present invention induces C57BL / 6 alkaloids by DSS The colitis model was established in mice. The total alkaloids of Jingdongshan orange can effectively inhibit the expression of related inflammatory factors in the colon tissue induced by DSS, and can increase the activity of related oxidases in the colon tissue. The total alkaloids of Jingdongshan orange have a good development and application prospect in the preparation of drugs for the prevention and treatment of inflammatory bowel disease.

Description

technical field [0001] The invention relates to the application of total alkaloids of Jingdongshan orange in the preparation of medicines for preventing and treating inflammatory bowel disease, and belongs to the technical field of medicines. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a non-specific intestinal disease characterized by chronic inflammation and ulceration. At present, there is no effective cure. It is a chronic disease of the digestive system. , weight loss, purulent mucus stool, and tenesmus are the main symptoms; the lesion of the disease is mainly the mucosa and submucosa of the colon, mainly ulcers and edema, mostly involving the rectum, distal colon or even the entire colon. It is the result of multi-factors, multi-mechanisms, and multi-links. The course of the disease is long, the disease is prone to relapse and delay in healing, and it can cause serious complications and the possibility of cancer. It ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/24A61P1/00
CPCA61K36/24A61K2236/333A61K2236/55A61P1/00
Inventor 程桂广杨美莲何舒悦曹建新赵天瑞
Owner 云南聚和生物科技有限公司
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