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Use of palm fruit extract

A technology of extract and palm fruit, which is applied in the field of food or medicine, can solve problems such as no literature reports, achieve the effect of increasing added value, enriching raw materials, and reducing costs

Inactive Publication Date: 2019-01-11
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports in the literature on the use of different polyphenol extracts from palm fruit for pancrelipase and α-glucosidase inhibitors.

Method used

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  • Use of palm fruit extract
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  • Use of palm fruit extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Preparation of polyphenol extracts in different states of palm fruit

[0018] Weigh 30g of degreased palm fruit powder (degrease with petroleum ether according to conventional methods), add methanol-acetone mixture (70% mass concentration methanol solution and 70% mass concentration acetone solution by volume) according to the ratio of solid to liquid g / mL1:5 ratio of 1:1), ultrasonic extraction, ultrasonic power is 200W, time is 30 minutes, and then filtered, the filter residue is extracted twice again, the combined filtrate is collected and the organic reagent is removed by rotary evaporation, and the aqueous phase is collected for Extraction of free and esterified polyphenols.

[0019] Extraction of free polyphenols: first adjust the pH of the aqueous phase to 2 with 6mol / L hydrochloric acid, then extract with ether-ethyl acetate mixture (1:1 v / v) until the extract is colorless, then combine the extracts And rotary evaporating and lyophilizing to obtai...

Embodiment 2

[0022] Example 2: Identification of Extract Components

[0023] The material composition analysis in the extract in Example 1 was identified by HPLC-ESI-HRMS / MS, as follows: the separation column was a 1.8×100 mm, 2 μm C18 column, the aqueous phase A was 2% formic acid water, and the organic phase B 2% acidified acetonitrile, separation and elution gradient: 0-2 min, 5% B; 2-10 min, 5-50% B; 10-14 min, 50% B; 14-15 min, 50-5% B; 15-20 min, 5% B; injection volume 3 μL, flow rate 0.2 mL / min. The mass spectrometry data were collected by a Q-Exactive Orbitrap mass spectrometer, and the relevant parameters were: spray voltage 3.3 kV, ion transfer tube temperature 320 °C, heater temperature 350 °C, sheath gas flow rate 32 L / min, auxiliary gas flow rate 8 L / min, purge gas flow rate 4L / min, full scan range 50–1000m / z. By comparing the mass spectrometry data with relevant databases and standards, a total of more than ten substances were identified, most of which were phenolic compoun...

Embodiment 3

[0026] Embodiment 3: Determination of total phenolic content of different extracts of palm fruit

[0027] The determination was carried out by the Folinol method, and the specific operation was as follows: each extract was dissolved in methanol and diluted to 1.0 mg / mL; 1 mL of the extract solution and Folinol reagent were mixed; after standing for 1 minute, 1.5 mL of Na 2 CO 3 ; Dilute to 10 mL with distilled water. 70°C water bath for 10 minutes, after cooling to room temperature, measure the absorbance value at 765nm, the content of total phenols is calculated by gallic acid calibration; the results are as follows figure 1 As shown, the total phenolic content of each extract is (mg gallic acid / g palm fruit): free polyphenol 81.20±6.07 mg / g, esterified polyphenol 163.06±3.06 mg / g, bound polyphenol 220.49± It can be seen from the results that the phenolic substances in palm fruit mainly exist in the form of bound polyphenols.

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Abstract

The present invention discloses the use of palm fruit extract in inhibiting pancreatic lipase and alpha-glucosidase in medicine or health food, belonging to the technical field of food or medicine. Asthe palm fruit is taken as a raw material, different extracts of the palm fruit are obtained by extracting with an organic reagent or a mixed solution of water and an organic reagent, and the extracts of the palm fruit are purified by in vitro pancreatic lipase and alpha; glycosidase activity assay shows that all extracts could effectively inhibit pancreatic lipase and alpha-glucosidase activity,and reduce fat and glucose absorption, therefore, the invention can be used for preparing health food and medicine related to lipid reduction, weight loss, blood sugar reduction and other diseases.

Description

technical field [0001] The invention relates to a new application of palm fruit extract in food and medicine, and belongs to the technical field of food or medicine. Background technique [0002] With the continuous improvement of people's material life, unhealthy high-sugar and high-fat diet and bad living habits induce the occurrence of various chronic diseases. Excessive intake of lipids and severe lack of exercise lead to fat deposition in various parts of the body, which gradually evolves into obesity. At present, obesity is increasing at an alarming rate, endangering the health of the population in countries with high-fat eating habits, and has become a worldwide health topic. Obesity is related to many chronic diseases, such as diabetes, fatty liver and cardiovascular diseases, etc. According to the World Health Organization, the death toll of obesity is much higher than that of light-weight people, and about 65% of people in developed countries are obese crowd. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/889A61P3/04A61P3/10A23L33/105
CPCA61K36/889A61P3/04A61P3/10A23L33/105A61K2236/55A61K2236/51A61K2236/39A61K2236/333A23V2002/00A23V2200/328A23V2200/332A23V2250/21
Inventor 蔡圣宝周洁心王春丽曾内辉马爽杨俊娴
Owner KUNMING UNIV OF SCI & TECH
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