Application of Isaria javanica in control of coccoiol

A technology of Isyria clavus and scale insects, applied in the fields of application, insecticide, animal repellent, etc., to achieve the effects of no pollution to the environment, easy preparation, and good market application prospects

Active Publication Date: 2019-01-29
广州市林业和园林科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of the scale insect control technologies such as the existing Egyptian phagocytosis and Pyococcus hibiscus, and to provide a kind of important Egyptian phagocytosis entomopathogenic fungus, i.e. I. javanica ( Isariajavanica), which has high pathogenicity to P. aegypti, can be used as a living biopesticide with high pathogenicity to Homopteran insects such as P. aegypti and Pythococcus hibiscus, and has high efficiency, environmental friendliness, It has the advantages of not easy to produce drug resistance, etc., and has a good application prospect in the prevention and control of the Egyptian scorpion

Method used

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  • Application of Isaria javanica in control of coccoiol
  • Application of Isaria javanica in control of coccoiol
  • Application of Isaria javanica in control of coccoiol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation of Corynespora javanicus strain IJID003

[0037] A highly pathogenic fungus was obtained from the naturally susceptible Pygmycidae from Bailan in Yuexiu Park, Guangzhou City, Guangdong Province. The method is as follows:

[0038] (1) Separation of fungi

[0039] The hyphae were picked from the susceptible Pseudococcidae, inoculated on the PDA medium and cultured at 27±1℃ for 7 days. The hyphae were picked from the center of the colony, purified and cultured on the PDA medium, and the purification culture was repeated 3 times, and placed in a refrigerator at 4°C for later use.

[0040] (2) Fungus tieback experiment

[0041] Cultivate the isolated fungi on a PDA plate for 7-15 days. After the bacteria are formed, add 0.02% Tween-80 sterile water to wash the spores, stir for 15 minutes on a magnetic stirrer, and adjust the spore concentration to 1 with a hemocytometer. ×10 7 The spore suspension was prepared with a small hand sprayer, and sprayed evenly on the ...

Embodiment 2

[0044] Example 2 Screening of Corynespora javanicus strain IJID003

[0045] Entomopathogenic fungi have diversity in genetics, ecology, and biology. Among them, different strains of fungi show significant differences in their pathogenicity to target pests. The selection and acquisition of high-yield and high-quality strains is the primary prerequisite for achieving better control effects. The selection of excellent strains mainly considers three indicators, namely, spore production, colony growth rate, and pathogenicity. The present invention uses these three indicators as the main basis to screen excellent highly pathogenic strains.

[0046] (1) Material preparation

[0047] Purified IJID001, IJID002, IJID003, IJID004, IJID005, IJID006, IJID007, IJID008, 8 isolates, were cultured on a PDA plate in a constant temperature incubator at 27±1°C.

[0048] (2) Test insects and host plants

[0049] The Egyptian Pseudococcidae comes from the successive breeding population on the greenhouse w...

Embodiment 3

[0064] Example 3 Identification of strain IJID003

[0065] (1) Morphological characteristics of strain IJID003

[0066] Inoculate the strain IJID003 into the center of the PDA medium plate, place it at 27±1℃ for constant temperature culture, observe the growth of the colony every day and record the colony color and shape. Inoculate the strain IJID003 into the center of another PDA medium plate, and at the same time insert the sterilized cover glass (1cm×1cm) obliquely into the medium about 1cm away from the inoculation point, and place it at 27±1℃ for 5 days. Allow the hyphae to extend to the cover glass, take out the cover glass and place it under an optical microscope to observe the hypha and spore morphology.

[0067] Morphological characteristics of strain IJID003 ( figure 1 with figure 2 ): On the PDA plate, the colonies are round, with a diameter between 49mm and 55mm, with concentric ring patterns, and a ravine is formed on the medium, and the texture is thick and fluffy. T...

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Abstract

The invention discloses application of Isaria javanica in control of coccoiol. It is found that Isaria javanica has a good pathogenic control effect on Icerya aegyptiaca (Douglas), Phenacoccus solenopsis Tinsley and other coccoiol pests, can be used as living coccoiol biological pesticide with a high pathogenicity of coccoiol, and has a good application prospect in biological control of coccoiol insects. Moreover, Isaria javanica is an entomopathogenic fungus, conidial suspension of Isaria javanica can directly applied to pest bodies, and when compared with current chemical control agents, high efficiency, green and environmental friendliness, environmental protection, high persistence, no easy production of chemical resistance, no pollution to the environment, no residues and other advantages are achieved; and a good market application prospect is achieved when a developed biocontrol preparation is applied to biological control of the coccoiol insects.

Description

Technical field [0001] The invention belongs to the technical field of pest biological control. More specifically, it relates to the application of Corynespora javanica to control Homoptera insects of scale insects. Background technique [0002] Homoptera is one of the common forest pests, such as Icerya aegyptiaca (Douglas), Phenacoccus solenopsis Tinsley and other scale insect pests. Iceryaaegyptiaca (Douglas) is an important pest that harms forestry and garden plants. It is mainly distributed in Guangdong, Fujian, Zhejiang, Taiwan and other places in my country. It mainly harms forestry and pests such as white orchid, lotus magnolia, and manglietia. Garden plants. As early as the 1980s, this insect was listed as one of the dangerous forestry and garden pests in Guangzhou. The female adults and nymphs of the Egyptian worms mainly harm the leaf buds, twigs and branches of plants. They gather in groups on the back of the leaves or on the twigs to absorb plant sap, ranging from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04
CPCA01N63/30
Inventor 邓嘉茹黄华枝毕可可孙龙华张劲蔼唐立鸿吴超候亚会霍立志阮琳
Owner 广州市林业和园林科学研究院
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