A kind of metal-organic complex nanomaterial and its preparation method and application

A technology of nanomaterials and complexes, applied in the field of antitumor drug preparation, can solve problems such as limiting transformation

Active Publication Date: 2020-10-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how to release the insoluble complex NQ+Cu(II) to the tumor site through systemic administration is a great challenge, which severely limits its translation to clinical applications.

Method used

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Embodiment 1

[0044] 1. Preparation of nanomedicine

[0045] (1) Solution preparation

[0046] Aqueous solution of copper nitrate: Weigh 0.75 g of copper nitrate and dissolve it in 40 mL of deionized water to prepare an aqueous solution of copper nitrate with a concentration of 100 mM.

[0047] 5-nitro-8-hydroxyquinoline acetone solution: Weigh 0.38g 5-nitro-8-hydroxyquinoline and dissolve it in 40mL acetone to prepare 5-nitro-8-hydroxyquinoline acetone with a concentration of 50mM solution.

[0048] (2) Add 20 mg of bovine serum albumin to 5 mL of deionized water, and then add 20 μmol of sodium hydroxide, so that the concentration of sodium hydroxide in the system is 4 mM. Then, 0.1 mL of copper nitrate aqueous solution and 0.5 mL of 5-nitro-8-hydroxyquinoline acetone solution were added dropwise at a rate of 100 μL / min at the same time, and stirred while dropping. After the dropwise addition, the reaction was carried out at room temperature for 3 hours.

[0049] (3) Dialysis (Mw=10000...

Embodiment 2

[0066] Nano-medicines were prepared according to the reaction conditions in Table 1, and the process flow was referred to in Example 1. Table 1 shows the particle size results of the obtained products.

[0067] Table 1

[0068]

[0069] Note: In the above table, BSA: bovine serum albumin; NaOH: sodium hydroxide; Cu(NO 3 ) 2 : copper nitrate; NQ: 5-nitro-8-hydroxyquinoline.

[0070] It can be seen from the above table that when the amount of BSA or NaOH is too much, the size of the nanomedicine is too large. Nanoparticles that are too large cannot be enriched in tumor tissue through the EPR effect, and are easily trapped by the reticuloendothelial system. Therefore, they are not suitable for use as the metal-organic complex nanomedicine and tumor treatment of the present invention.

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Abstract

The invention discloses a metal-organic matter complex nanometer material as well as a preparation method and applications of the metal-organic matter complex nanometer material, and belongs to the technical field of preparation of antitumor medicines. The preparation method comprises the following steps: adding bovine serum albumin into an alkaline solution, simultaneously dropwise adding coppernitrate solution and 5- nitro-8-hydroxyquinoline solution, and carrying out reaction under the room temperature, so that the metal-organic matter complex nanometer material is obtained through productself-assembly. Through the simple and pollution-free preparation route, the prepared nanometer material has the advantages of good stability, long in-vivo circulation time, good targeting property and biological compatibility, small toxic and side effects, and the like. The provided nanometer material can be applied to tumor treatment as a nanometer medicine, and gathers in the tumor tissue through the EPR effect and the receptor-mediated endocytosis, so that the treatment effect of the metal-organic matter medicine for tumors is improved, and the systemic administration of the metal-organicmatter medicine can be realized.

Description

technical field [0001] The invention relates to the technical field of preparation of antitumor drugs, in particular to a nanometer material based on a 5-nitro-8-hydroxyquinoline copper complex and a preparation method and application thereof. Background technique [0002] In recent years, the morbidity and mortality of malignant tumors are on the rise, and cancer has become the number one cause of death among urban and rural residents in my country. Small-molecule drug therapy is still one of the main means of clinical tumor treatment, but there are many negative features, such as: low solubility in water, difficult to use directly; plasma half-life is too short, continuous repeated administration is necessary; poor targeting, There are toxic and side effects on the body; tumor resistance to chemotherapy drugs, etc. [0003] Antitumor drugs based on transition metal elements, especially platinum, such as cisplatin, have shown significant effects in clinical cancer treatmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K31/555A61P35/00
CPCA61K31/555A61K47/42A61P35/00
Inventor 唐建斌胡丁徐红霞申有青刘祥瑞周珠贤
Owner ZHEJIANG UNIV
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