Cryoprotectant capable of being used for hematopoietic stem cells or lymphocytes and cryopreservation method

Abstract

The invention discloses cryoprotectant capable of being used for hematopoietic stem cells or lymphocytes and a cryopreservation method. The cryoprotectant is prepared from dimethyl sulfoxide (DMSO), human serum albumin and normal saline in proportioning and blending modes, and the volume ratio of the DMSO to the human serum albumin to the normal saline is 1 to 2 to 2. The invention further comprises a method for cryopreservation of the hematopoietic stem cells or the lymphocytes by applying the cryoprotectant, and the method comprises the following steps of (1) cryoprotectant preparation, (2)cryopreservation subpackage, (3) heat sealing and (4) gradient cryopreservation. A cell culture medium and animal serum are not added into the cryoprotectant, thus infusion can be directly conducted,and no adverse reaction application is achieved; compared with traditional cryoprotectant, allergen bringing is avoided, and safety is higher; the cryoprotectant is high in cryopreservation efficiency, the activity of the stem cells after cryopreservation resuscitation is greater than 85%, the activity of the lymphocytes is greater than 90%, and the manufacturing and using costs of the cryoprotectant are lowered on the basis that the physiological properties of the cryopreserved cells are effectively maintained. The cryopreservation method is easy and convenient to operate, required equipmentis simple, and the cryopreservation method is suitable for being applied in different levels of hospitals.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More

AI Technical Summary

Benefits of technology

This technology allows for quicker cool down on both hormone sensitive (hypophyseal) and nonreactive types of blood cells that may have been damaged by freeze drying techniques such as lysozyme preserving methods. Additionally, this technique helps preserve these important properties while also making them easier to use at home instead of requiring expensive facilities like liquid nitrogen gas cylinders. Overall, this new approach provides an effective way to store living tissue samples safely over long periods of times.

Problems solved by technology

Technical Problem addressed in this patented technical problem relates to improving the efficacy of cryogenesis techniques during medical treatments like chemoradiology or immunodeficiency syndrome due to poor recovery rates caused by low levels of active ingredients associated with these procedures. Current methods involve multiple steps involving tissue culture media, handling, preserving them under special conditions, and then delivering them into patient' s body through postal delivery systems. However, current methods suffer drawbacks including lack of effective purification protocols, difficulty in obtaining suitable compositions containing certain substances, excessive costs involved, complex processes requiring expensive equipment, etc., making their overall benefits difficult to achieve.

Images