A kind of heavy metal resistant microorganism and its application
A technology of heavy metals and microorganisms, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of lack of microorganisms that can effectively repair soil or water with multiple heavy metals, and lack of microorganisms that are polluted by heavy metals in water and soil.
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Embodiment 1
[0054] (1) Dissolve 5g of manganese ore tailings in 20mL of 0.9% sterile NaCl solution, shake in a shaker at 100rpm for 10min at 25°C, and let stand for 20min;
[0055] (2) Take 1mL of the suspension and add it into sterile water containing Mn 4000mg / L, Cd 100mg / L, Pb 100mg / L, Zn 100mg / L and Cu 100mg / L, at 25°C and 100rpm in a shaker Oscillation 6h;
[0056] (3) Dilute 1mL of the reaction solution for 10 3 times, take 200µL of the culture solution and spread it on the PDA solid medium, and culture it for 2-7 days at 25°C;
[0057] (4) Pick a single colony, inoculate it into PDA liquid medium, and cultivate it for 2-5 days at 25°C;
[0058] Aspirate 100 µL of the culture solution and inoculate it into the PDA liquid medium containing different concentrations of Mn, Cd, Pb, Zn, or Cu to measure the resistance to Mn, Cd, Pb, Zn, and Cu.
[0059] The screened microorganism Sporobolomyces carnicolor MWT-01, such as figure 1 , figure 2 shown.
[0060] The Neighbor-Joining phy...
Embodiment 2
[0064] (1) Select microorganisms with excellent resistance to Mn, Cd, Pb, Zn, and Cu, culture them at 25°C for 2-7 days, and carry out microbial amplification;
[0065] (2) Add 98mL of 2mL bacterial solution containing 4g / L kaolin and 0.5g / LCaCl 2 in solution;
[0066] (3) After shaking repeatedly for 10-20 times, let it stand for 5 minutes, take 5mL supernatant and measure the OD value at 550nm to detect the microbial flocculation rate. Such as Figure 5 It can be seen from the figure that the flocculation rate of Sporobolomyces carnicolor MWT-01 increases rapidly and reaches the maximum during the 5-7 day growth period.
[0067] (4) Select microorganisms with high flocculation rate to carry out the heavy metal resistance experiment again. The obtained microorganisms Sporobolomyces carnicolor MWT-01 Mn resistance reaches 10000mg / L, Cd resistance reaches 600mg / L, Pb resistance reaches 800mg / L, Zn The resistance reaches 1000mg / L, and the Cu resistance reaches 1500mg / L, such ...
Embodiment 3
[0069] (1) Sporobolomyces carnicolor MWT-01 was cultured in PDA liquid medium for 7 days.
[0070] (2) Add 5mg / LCaCl to the PDA liquid medium containing Sporobolomyces carnicolor MWT-01 cells 2 .
[0071] (3) Add the culture medium containing Sporobolomyces carnicolor MWT-01 to the water body containing 150mg / L Mn, 100mg / L Cd, 100mg / L Pb, 50mg / L Zn and 50mg / L Cu, at a dosage of 2.5mL / L, The concentration of Sporobolomycescarnicolor MWT-01 is 2*10 9 cfu / ml.
[0072] (4) The coagulation removal rates of Mn, Cd, Pb, Zn, and Cu are 82.1%, 82.7%, 92.3%, 100%, and 100%, respectively.
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