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Caper extract, and preparation method and application thereof

A technology of capers and extracts, applied in the field of capers extracts, can solve the problems of complex extract components, cumbersome methods, crude extracts, etc., and achieve good anti-rheumatoid arthritis effect

Inactive Publication Date: 2019-02-26
SHANGHAI INST OF PHARMA IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a caper extract, its Preparation method and application

Method used

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  • Caper extract, and preparation method and application thereof
  • Caper extract, and preparation method and application thereof
  • Caper extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Get 2.0kg of Capers medicinal material (place of origin: Turpan), add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, then add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, combine the two extracts, Concentrate under reduced pressure at 60°C until the density of the extract is 1.15g / ml, slowly add an appropriate amount of 95% ethanol to ethanol concentration of 70%, and let stand overnight. Concentrate the supernatant under reduced pressure at 60°C until there is no alcohol smell, add an appropriate amount of aqueous solution to dissolve, and apply D101 macroporous adsorption resin at a flow rate of 1.5BV / h. After the sample is loaded, it is washed with water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, each gradient solvent was eluted for 5 column volumes, the eluate of 70% ethanol was collected, concentrated under reduced pressure at 60°C until there was no alcohol s...

Embodiment 2

[0038]Get 2.0kg of Capers medicinal material (place of origin: Turpan), add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, then add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, combine the two extracts, Concentrate under reduced pressure at 60°C until the density of the extract is 1.18g / ml, slowly add an appropriate amount of 95% ethanol to ethanol concentration of 70%, and let stand overnight. Concentrate the supernatant under reduced pressure at 60°C until there is no alcohol smell, add an appropriate amount of aqueous solution to dissolve, and apply D101 macroporous adsorption resin at a flow rate of 2.0BV / h. After the sample is loaded, it is eluted with water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, each gradient solvent was eluted with 4.5 column volumes, the eluate of 70% ethanol was collected, concentrated under reduced pressure at 60°C until there was no alcohol...

Embodiment 3

[0040] Get 2.0kg of Capers medicinal material (place of origin: Turpan), add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, then add 8 times the amount of aqueous solution for reflux extraction for 2 hours, filter the extract, combine the two extracts, Concentrate under reduced pressure at 60°C until the density of the extract is 1.10g / ml, slowly add an appropriate amount of 95% ethanol to ethanol concentration of 70%, and let stand overnight. Concentrate the supernatant under reduced pressure at 60°C until there is no alcohol smell, add an appropriate amount of aqueous solution to dissolve, and apply D101 macroporous adsorption resin at a flow rate of 2.0BV / h. After the sample is loaded, it is eluted with water, 30% ethanol, 70% ethanol and 95% ethanol were eluted, each gradient eluent was eluted in 5.5 column volumes, the eluate of 70% ethanol was collected, concentrated under reduced pressure at 60°C until no alcohol smell was o...

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Abstract

The invention discloses a caper extract, and a preparation method and application thereof. The preparation method comprises the following steps: loading an aqueous solution of a crude caper extract onto macroporous adsorption resin for chromatography; then carrying out elution by using water, ethanol with a concentration of 20%-30% and ethanol with a concentration of 65%-75%, successively; concentrating an eluate obtained after elution by using the ethanol with a concentration of 65%-75% until no alcohol smell exists, and mixing the obtained concentrate with water; and carrying out extractionby using ethyl acetate, and taking the part of an ethyl acetate solution, wherein the above percentages are volume percentages. The extract prepared by using the preparation method in the invention contains relatively simple compound components and is significantly enhanced in the activity of resisting rheumatoid arthritis.

Description

technical field [0001] The present invention relates to capers extract, its preparation method and application. Background technique [0002] Capers (also known as: wild watermelon, mouse melon) (Capparis spinosa Linn.) is a small perennial vine and semi-shrub of the family Capparaceae (Capparaceae), mainly distributed in the warm climates of the Mediterranean Sea, Central Asia and West Asia. In temperate regions, it grows in arid and desert soils. The leaves, roots, root bark, flowers, fruits and seeds of capers are used for the treatment of common diseases among the Uighur people in Turpan, among which the fruits, leaves, roots and root bark are widely used to treat acute and chronic arthritis, rheumatism and shoulder pain. Zhou Yan. Capers are used as a traditional medicine in many countries. Its leaves, fruits and root bark have the effects of expelling wind, dehumidification, clearing the nose, stopping stasis and swelling, relieving pain and promoting blood circulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P29/00A61P19/02
CPCA61K36/185A61K2236/331A61K2236/55
Inventor 吴彤沈龙海周海凤乐心逸王娴娴李默影张蓓
Owner SHANGHAI INST OF PHARMA IND CO LTD