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Euphausia superba-originated antihypertensive tripeptide and application thereof

An Antarctic krill and blood pressure-lowering technology, which is applied in the fields of application, tripeptide ingredients, and medical preparations containing active ingredients, etc., to achieve the effect of reasonable design and convenient operation

Active Publication Date: 2019-03-01
中科利君股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The applicant found that the preparation of Antarctic krill blood pressure-lowering peptide by enzymolysis technology using Antarctic krill as raw material is in a blank stage

Method used

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  • Euphausia superba-originated antihypertensive tripeptide and application thereof
  • Euphausia superba-originated antihypertensive tripeptide and application thereof
  • Euphausia superba-originated antihypertensive tripeptide and application thereof

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Embodiment 1

[0041] An antihypertensive tripeptide derived from Antarctic krill, containing an antihypertensive tripeptide with an amino acid sequence of Phe-Ala-Ser. The Antarctic krill hypotensive tripeptide Phe-Ala-Ser (FAS) provided by the present invention has significant inhibitory effect on its ACE, half maximal inhibitory concentration (IC 50 ) is 0.152mg / mL, the functional evaluation results of Phe-Ala-Ser (FAS) to human umbilical vein endothelial cells (HUVEC) show that: at the concentration of 100-500μM, Phe-Ala-Ser (FAS) is effective for HUVEC cells It has no obvious toxic effect, and can promote the release of endogenous relaxation factor (NO) in HUVEC cells and inhibit the production of endothelin (ET-1). Therefore, the result of the effect of Phe-Ala-Ser (FAS) on ACE and HUVEC cells shows that it has good activity and can be used as a promising antihypertensive peptide for antihypertensive drugs, health care products and special medical food.

[0042] The peptide was determ...

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Abstract

The invention provides a euphausia superba-originated antihypertensive tripeptide and application thereof and belongs to the technical field of antihypertensive oligopeptides. The euphausia superba-originated antihypertensive tripeptide has an amino acid sequence of Phe-Ala-Ser with an ESI-MS (electrospray ionization mass spectrometry) measured molecular weight of 323.35 Da. The preparation methodof the euphausia superba-originated antihypertensive tripeptide comprises homogenizing euphausia superba, adding in distilled water and proteases for enzymolysis in an environment of 45-55 DEG C for5.5-6.5 h to obtain an enzymolyzed euphausia superba solution; performing ultrafiltration, anion exchange chromatography, gel column chromatography and reversed-phase high-performance liquid chromatography to prepare the euphausia superba-originated antihypertensive tripeptide. The euphausia superba-originated antihypertensive tripeptide achieves significant inhibiting effects on ACE (angiotensinconverting enzyme), is free from toxic effects on human umbilical vein endothelial cells (HUVEC), promotes releasing of endogenous relaxing factors in HUVEC and inhibits endothelin, thereby being capable of serving as antihypertensive peptide fragments with a good developing prospect for being applied to antihypertensive medicines, healthcare products and special medical foods.

Description

technical field [0001] The invention belongs to the technical field of hypotensive oligopeptides, in particular to a hypotensive tripeptide derived from Antarctic krill and its application. Background technique [0002] Hypertension refers to an increase in systemic arterial blood pressure (systolic blood pressure and / or diastolic blood pressure) as the main feature (systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg), which may be accompanied by heart, brain, kidney and other organ functions Or clinical syndrome of organic damage. Hypertension is a common disease and frequently-occurring disease. The disease generally has a slow onset, and patients often have no symptoms in the early stage, or only have symptoms such as dizziness, headache, palpitations, and tinnitus. If it is not treated properly, it will become a more serious complication of hypertension such as stroke, myocardial infarction and renal failure. Angiotensin-converting enzyme (ACE) i...

Claims

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Application Information

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IPC IPC(8): C07K5/087C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16C12P21/06A61K38/06A61P9/12A23L33/18
CPCA23V2002/00A23V2200/326A23V2250/55A61K38/00A23L33/18A61P9/12C07K5/0812C12P21/06
Inventor 王斌陶静赵玉勤
Owner 中科利君股份有限公司
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