Method for judging platelet aggregation rate measurement results and calibration method for platelet aggregation meter

Abstract

The invention provides a method for judging the measurement result of platelet aggregation rate, comprising the following steps: selecting a normal whole blood sample, measuring the transmittance PPP0 of platelet-poor plasma and the transmittance PRP0 of platelet-rich plasma, and calculating the ratio of the transmittance of the whole blood sample Difference P0=PPP0-PRP0; select the whole blood sample to be tested, measure the platelet-poor plasma transmittance PPP and platelet-rich plasma transmittance PRP, and calculate the difference between the transmittance of the tested sample P=PPP-PRP; calculate the measured The platelet separation index DI=P / P0=(PPP-PRP) / (PPP0-PRP0) of the sample; the platelet aggregation rate of platelet-rich plasma is measured by a platelet aggregometer; according to the platelet separation index DI of the tested sample, the determination measurement Whether the platelet aggregation rate is reliable. The invention reliably judges the measurement result of the platelet aggregation rate of the tested sample by referring to the platelet separation index of the tested sample.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for judging a platelet aggregation rate measurement result and a calibration method suitable for a platelet aggregation meter. Background technique [0002] Optical turbidimetry is a more practical and easy-to-promote clinical method for detecting platelet aggregation. This method is simple to operate, inexpensive, and well correlated with clinical events. It is the gold standard for detecting platelet aggregation. [0003] Platelet-rich plasma (PRP) is a suspension of platelets in plasma. The platelets are evenly dispersed, and the turbidity is proportional to the number of platelets contained in the plasma. When the platelet aggregation inducer is added, the inducer and platelet The above receptors bind, the platelets are activated, and the platelets deform to further release endogenous substances, resulting in aggregation and increased light transmittance. The higher...

AI Technical Summary

Problems solved by technology

[0005] The platelet aggregation rate measured by the existing method is stable when the platelet concentration in the blood sample reaches a certain level, but if the platelet concentration is less than a certain value, the aggregation rate will also decrease, making the instrument report The aggregation rate is unreliable and cannot accurately reflect the platelet aggregation function. At present, before performing the platelet aggregation function test, hospitals generally use a hematology analyzer to count the platelets first, so as to measure the platelet concentration. If the measured value is higher than a certain When the value is higher, the platelet aggregation rate result obtained from the measurement is a reliable value, otherwise, it will report a doubtful result

This method is cumbersome to operate, and requires the use of extra blood analyzers and other instruments, and it is impossible to obtain the results at one time when measuring the platelet aggregation rate.

Images