A kind of tobacco kup6 protein and its coding gene and application

Abstract

The invention relates to the field of genetic engineering, and specifically discloses a tobacco KUP6 protein, its coding gene and its application. The present invention provides the KUP6 gene isolated from tobacco for the first time. The nucleotide sequence of the KUP6 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein KUP6 encoded by it is shown in SEQ ID NO.1. After functional verification, After the KUP6 gene provided by the invention is transferred into the potassium absorption-deficient yeast mutant strain R5421, the recombinant yeast expressing the KUP6 gene has the functions of potassium ion absorption and transport. It shows that the KUP6 gene provided by the present invention has the function of promoting the absorption and transport of potassium ions.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a potassium transporter KUP6 from tobacco and its coding gene and application. Background technique [0002] Potassium transporter is a carrier protein that can transport potassium ions into cells when the external potassium ion concentration is extremely low. Potassium transporters are usually some transmembrane proteins, which complete the transmembrane transport of potassium ions through conformational changes. It is an active transport process that requires ATP to provide energy, usually K + / H + or K + / Na + Collaborative transport. [0003] Existing technology studies on potassium transporter genes are extensive in the model plant Arabidopsis thaliana. For example, studies have shown that Arabidopsis mutant kup6 has a lower survival rate under drought stress than wild-type plants, while 35S::KUP6 overexpression The drought tolerance of transgenic plants was significa...

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Problems solved by technology

[0003] Existing technology studies on potassium transporter genes are extensive in the model plant Arabidopsis thaliana. For example, studies have shown that Arabidopsis mutant kup6 has a lower survival rate under drought stress than wild-type plants, while 35S::KUP6 overexpression The drought tolerance of transgenic plants was significantly enhanced (Shabala et al., 2007); while the mutant seedlings of KUP7 gene exhibited a low-potassium-sensitive phenotype of yellowing leaves under low-potassium stress, and the potassium ions in the roots The content decreased significantly, and the loss of KUP7 gene function reduced the ability of Arabidopsis seedlings to absorb potassium under low potassium conditions, and the concentration of potassium ions in xylem juice decreased (Min et al., 2016)

[0004] Tobacco is a crop that consumes a lot of potassium. The potassium content of tobacco leaves is an important indicator to measure the quality of tobacco leaves. At present, there are few studies on potassium transporter genes in tobacco, and the function of tobacco KUP is unknown.

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