Device and method for detecting pesticide residue and nitrite based on scanning code type paper chip

Abstract

The invention relates to a device and a method for detecting pesticide residue and nitrite based on a scanning code type paper chip by adopting a microfluidic technology and an image processing technology to identify, detect and record the pesticide residue and nitrite harmful substances. The method includes, arranging a plurality of layers of microfluidic paper chips inside a heating chip detection cavity, wherein a plurality of layers of the microfluidic paper chips consist of five layers which are glued together from top to bottom, each layer has a chip bar code detection areal adding, by adropper, mixed liquid into a chip sample inlet drop by drop; showing, by the chip bar code detection area of each layer, different colors according to a composition of the sample mixed liquid; imaging and scanning, by intelligent mobile equipment, a color development result of a plurality of layers of the microfluidic paper chips to obtain a detection result. The method applies an inhibition principle of organophosphorus pesticides on acetylcholine esterase and a diazonium coupling reaction of aminobenzenesulfonic acid and N-1-naphthylethylenediamine dihydrochloride under an acidic condition,and integrates automatic sample introduction, intelligent heating, reaction, color development and result analysis, recording and processing.

Description

technical field [0001] The invention relates to a portable device for identifying, detecting and recording pesticide residues and nitrite harmful substances by adopting microfluidic technology and image processing technology. Background technique [0002] At present, chemical fertilizers and pesticides are still the main means to prevent crop diseases and increase nutrients in crops and soil. However, pesticide residues are extremely harmful to the human body, and residual toxins are not suitable for detection. Pesticide poisoning can cause cancer and death. It is widely used in water and Chemical substances remaining in the soil have also caused a series of environmental pollution problems. Nitrogen-containing fertilizers play a significant role in improving soil nutrients, and excessive or excessive use of nitrogen fertilizers is not only prone to water pollution, but also to a significant increase in nitrate and nitrite content in agricultural products. The use of nitrit...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects of the traditional detection card used in the detection of pesticide residues and nitrite, the present invention proposes an intelligent, automatic, high-throughput and portable detection of pesticide residues and nitrite based on a multi-layer paper-based microfluidic chip. The salt detection device and the detection method of the device realize multiple detections of a single index in one sample injection, so as to solve the problems of low automation, cumbersome detection procedures, large chance of detection results and low accuracy of the existing rapid detection cards , and more importantly, optimize the process of processing, analyzing and recording the experimental results, get rid of the process of strongly relying on manual analysis, processing and recording, and realize food safety anytime, anywhere, fast, convenient, automatic, intelligent and high accuracy detection records and analyze

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