A kind of nano colloidal gold and its preparation method and application
A nano-colloid and colloidal gold technology, which is applied in the field of immunoassay, can solve the problems of difficult control of the size of colloidal gold particles and uneven particle size distribution of colloidal particles, and achieve the effects of consistent uniformity, improved stability and stable performance
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Embodiment 1
[0036] The preparation method of nano colloidal gold described in the present embodiment, comprises the steps:
[0037] (1) Solution preparation
[0038] Accurately weigh 1.0g of chloroauric acid powder and dissolve it in ultrapure water. After completely dissolving, add ultrapure water to make up to 100mL, and prepare HAuCl with a final concentration of 1% (w / v). 4 Aqueous solution, store the prepared chloroauric acid solution in a brown reagent bottle, and store it at 4°C for later use;
[0039] Accurately weigh 1.0 g of trisodium citrate dihydrate solid powder, dissolve it in ultrapure water, add ultrapure water to 100 mL after complete dissolution, and make a final concentration of 1% (w / v) Na 3 C 6 h 5 o 7 The aqueous solution was filtered through a 0.22 μm filter membrane and stored at 4°C for later use;
[0040] Accurately weigh 710mg of disodium hydrogen phosphate solid powder, dissolve it in ultrapure water, add ultrapure water to make up to 100mL after completel...
Embodiment 2
[0044] The preparation method of nano colloidal gold described in the present embodiment, comprises the steps:
[0045] (1) Solution preparation
[0046] Accurately weigh 1.0g of chloroauric acid powder and dissolve it in ultrapure water. After completely dissolving, add ultrapure water to make up to 100mL, and prepare HAuCl with a final concentration of 1% (w / v). 4 Aqueous solution, store the prepared chloroauric acid solution in a brown reagent bottle, and store it at 4°C for later use;
[0047] Accurately weigh 1.0 g of trisodium citrate dihydrate solid powder, dissolve it in ultrapure water, add ultrapure water to 100 mL after complete dissolution, and make a final concentration of 1% (w / v) Na 3 C 6 h 5 o 7 The aqueous solution was filtered through a 0.22 μm filter membrane and stored at 4°C for later use;
[0048] Accurately weigh 1.9g of borax solid powder, dissolve it in ultrapure water, add ultrapure water to make up to 100mL after complete dissolution, and make a s...
Embodiment 3
[0052] The preparation method of nano colloidal gold described in the present embodiment, comprises the steps:
[0053] (1) Solution preparation
[0054] Accurately weigh 1.0g of chloroauric acid powder and dissolve it in ultrapure water. After completely dissolving, add ultrapure water to make up to 100mL, and prepare HAuCl with a final concentration of 1% (w / v). 4 Aqueous solution, store the prepared chloroauric acid solution in a brown reagent bottle, and store it at 4°C for later use;
[0055] Accurately weigh 1.0 g of trisodium citrate dihydrate solid powder, dissolve it in ultrapure water, add ultrapure water to 100 mL after complete dissolution, and make a final concentration of 1% (w / v) Na 3 C 6 h 5 o 7 The aqueous solution was filtered through a 0.22 μm filter membrane and stored at 4°C for later use;
[0056] Accurately weigh 530 mg of sodium carbonate solid powder, dissolve it in ultrapure water, add ultrapure water to make up to 100 mL after complete dissoluti...
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Abstract
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