Rapid propagation method for tissue culture of rubus sachalinensis adventitious buds
A technology for the proliferation and culture of Rubus sclerotiorum is applied in the field of rapid propagation of tissue culture of adventitious buds of Rubus sclerotiorum, which can solve problems such as lack of resources, and achieve the effects of protecting the ecological environment, protecting the environment and high survival rate.
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Embodiment 1
[0056] Embodiment 1, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye
[0057] 1. Selection of explants
[0058] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .
[0059] 2. Disinfection of explants
[0060] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with a concentration of 1% detergent (Guangzhou Liby Enterprise Group Co., Ltd., catalog number 6920174736939), and rinse with sterile water for 2 hours. On the ultra-clean workbench, soak for 30s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 8 minutes, washed with sterile water for 5 times, and the sterilized explants were obtained.
[0061] 3. Proliferation of adventitious ...
Embodiment 2
[0068] Embodiment 2, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye
[0069] 1. Selection of explants
[0070] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .
[0071] 2. Disinfection of explants
[0072] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with 1% detergent, and rinse with sterile water for 2.5 hours. On the ultra-clean workbench, soak for 25s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 7 minutes, washed with sterile water for 3 times, and the sterilized explants were obtained.
[0073] 3. Proliferation of adventitious buds
[0074] Peel off the last layer of scales from the sterilized explants in step 2, cu...
Embodiment 3
[0080] Embodiment 3, Rapid Propagation Method of Adventitious Bud Tissue Culture of Rubus kuye
[0081] 1. Selection of explants
[0082] In May and September 2017, at the Sumushan Forest Farm in Xinghe County, Ulanqab City, Inner Mongolia Autonomous Region, a robust and pest-free Rubus sakhalin daughter plant was selected, and the adventitious buds were removed completely, and stored at 4°C with moisture .
[0083] 2. Disinfection of explants
[0084] Peel off the outer scales of the adventitious buds obtained in step 1, wash twice with 1% detergent, and rinse with sterile water for 2 hours. On the ultra-clean workbench, soak for 25s with 75% ethanol solution, and then use 0.1% HgCl 2 The solution (adding 0.5-1 drop of Tween 80) was sterilized for 6 minutes, washed with sterile water for 4 times, and the sterilized explants were obtained.
[0085] 3. Proliferation of adventitious buds
[0086] Peel off the last layer of scales from the sterilized explants in step 2, cut ...
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