A method for rapid domestication of microorganisms capable of degrading 2,4,6-trichlorophenol

Abstract

The invention discloses a method for rapidly acclimating microorganisms capable of degrading 2,4,6-trichlorophenol based on co-metabolism technology, and belongs to the technical field of sewage biological treatment. The present invention uses SBR as the main reaction device, inoculates the activated sludge in the secondary sedimentation tank of urban sewage treatment plant, and adopts a two-stage method for domestication, that is, firstly, the sewage containing 2,4,6-trichlorophenol is introduced into the SBR for aeration Acclimatized to obtain activated sludge with a certain degradation ability, and then used sucrose as a co-substrate organic carbon source to introduce aerated domestication into the SBR reactor, and finally obtained aerobic sludge with a high ability to degrade 2,4,6-trichlorophenol activated sludge. The two-stage domestication method adopted in the present invention not only ensures the acquisition of microorganisms with higher 2,4,6-trichlorophenol degradation ability in a short period of time, but also reduces energy consumption, and has guiding significance for the treatment of phenol-containing wastewater.

Description

technical field

[0001] The invention belongs to the field of sewage biological treatment, in particular to a method for rapidly domesticating and degrading microorganisms that degrade 2,4,6-trichlorophenol based on co-metabolism technology, which can be used in environments containing 2,4,6-trichlorophenol Treatment of phenolic wastewater. Background technique

[0002] Trichlorophenol is mainly used in the industry to produce dye intermediates, pesticides and preservatives and other products. Due to non-standard discharge, it often appears in the wastewater of coking, printing and dyeing, wood preservation and other industries, and the existence of trichlorophenol has been detected in natural water bodies. As a typical and widely used industrial raw material, 2,4,6-trichlorophenol has "three-cause" effects on human body and aquatic animals and plants. It is a toxic organic pollutant and has been listed as a priority pollution control by the US Environmental Protection Agenc...

Examples

Embodiment 1

[0026] 1) Start the SBR reactor: The inoculated sludge of this system is the sludge from the secondary sedimentation tank of the urban sewage treatment plant. After inoculation, the sludge concentration of the SBR reactor is maintained between 4000 and 5000 mg / L.

[0027] 2) The runtime adjustment operation is as follows:

[0028] 2.1) The first stage: 0 to 36 days, the domestication system only introduces the distribution water containing 2,4,6-trichlorophenol, and 2,4,6-trichlorophenol is dosed according to the gradient concentration, that is, from 10mg / L Increase to 80mg / L in turn. After adding each concentration, no 2, 4, 6 trichlorophenols can be detected in the effluent until the degradation of microbial p-chlorophenols reaches the maximum, indicating that the first stage of domestication is completed; the second stage: 36 ~40 days, while maintaining the water inflow in the first stage, start to introduce sucrose to match the water, the sucrose concentration is 268mg / L, ...

Embodiment 2

[0033] The difference between this embodiment and Example 1 is that the concentration of sucrose in the influent is 357 mg / L. The degradation of 2,4,6-trichlorophenol and 2,4,6-trichlorophenol in the influent and effluent were detected respectively. The result is as figure 2 As shown in (b), after stable operation, the degradation amount of 2,4,6-trichlorophenol is 47mg / L.

Embodiment 3

[0035] The difference between this embodiment and Example 1 is that the concentration of sucrose in the influent is 447 mg / L. The degradation of 2,4,6-trichlorophenol and 2,4,6-trichlorophenol in the influent and effluent were detected respectively. The result is as figure 2 As shown in (c), after stable operation, the degradation amount of 2,4,6-trichlorophenol is 35mg / L.

technical field

[0001] The invention belongs to the field of sewage biological treatment, in particular to a method for rapidly domesticating and degrading microorganisms that degrade 2,4,6-trichlorophenol based on co-metabolism technology, which can be used in environments containing 2,4,6-trichlorophenol Treatment of phenolic wastewater. Background technique

[0002] Trichlorophenol is mainly used in the industry to produce dye intermediates, pesticides and preservatives and other products. Due to non-standard discharge, it often appears in the wastewater of coking, printing and dyeing, wood preservation and other industries, and the existence of trichlorophenol has been detected in natural water bodies. As a typical and widely used industrial raw material, 2,4,6-trichlorophenol has "three-cause" effects on human body and aquatic animals and plants. It is a toxic organic pollutant and has been listed as a priority pollution control by the US Environmental Protection Agenc...

Examples

Embodiment 1

[0026] 1) Start the SBR reactor: The inoculated sludge of this system is the sludge from the secondary sedimentation tank of the urban sewage treatment plant. After inoculation, the sludge concentration of the SBR reactor is maintained between 4000 and 5000 mg / L.

[0027] 2) The runtime adjustment operation is as follows:

[0028] 2.1) The first stage: 0 to 36 days, the domestication system only introduces the distribution water containing 2,4,6-trichlorophenol, and 2,4,6-trichlorophenol is dosed according to the gradient concentration, that is, from 10mg / L Increase to 80mg / L in turn. After adding each concentration, no 2, 4, 6 trichlorophenols can be detected in the effluent until the degradation of microbial p-chlorophenols reaches the maximum, indicating that the first stage of domestication is completed; the second stage: 36 ~40 days, while maintaining the water inflow in the first stage, start to introduce sucrose to match the water, the sucrose concentration is 268mg / L, ...

Embodiment 2

[0033] The difference between this embodiment and Example 1 is that the concentration of sucrose in the influent is 357 mg / L. The degradation of 2,4,6-trichlorophenol and 2,4,6-trichlorophenol in the influent and effluent were detected respectively. The result is as figure 2 As shown in (b), after stable operation, the degradation amount of 2,4,6-trichlorophenol is 47mg / L.

Embodiment 3

[0035] The difference between this embodiment and Example 1 is that the concentration of sucrose in the influent is 447 mg / L. The degradation of 2,4,6-trichlorophenol and 2,4,6-trichlorophenol in the influent and effluent were detected respectively. The result is as figure 2 As shown in (c), after stable operation, the degradation amount of 2,4,6-trichlorophenol is 35mg / L.

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