Modeling equipment, modeling method and application of a mouse intervertebral disc degeneration model

An intervertebral disc and modeling technology, applied in the field of intervertebral disc degeneration research, can solve the problems of unreliability, large specific gravity, long cycle, etc., and achieve the effect of increasing axial stress and accelerating the degeneration process.

Active Publication Date: 2021-06-04
南方医科大学第三附属医院(广东省骨科研究院)
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AI Technical Summary

Problems solved by technology

[0005] 1. In the mechanical induction model, the mechanical compression model requires professional equipment and is poor in operability, and the stress site is the tail vertebra of the mouse, so the simulation is insufficient. On the one hand, the instability model induces intervertebral disc degeneration for a long time, which is not easy On the other hand, fibrosis of the surrounding tissues after surgery may re-stabilize the unstable spine, which is unreliable and has poor repeatability; second, the structural damage model is fast and effective, but its simulation is poor , the transient structural changes caused by trauma account for a large proportion of pathological development; three: natural induction, although the simulation is good, but the cycle is long, the study proves that the intervertebral disc of 18-month-old mice has obvious degenerative changes, which brings Higher cost; 4: Spontaneous model, fast and has a certain degree of simulation but requires high technical level, complicated experimental process and persistent interference factors

Method used

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  • Modeling equipment, modeling method and application of a mouse intervertebral disc degeneration model
  • Modeling equipment, modeling method and application of a mouse intervertebral disc degeneration model
  • Modeling equipment, modeling method and application of a mouse intervertebral disc degeneration model

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Embodiment

[0050] Please refer to Figure 9-Figure 12 , the present embodiment provides a modeling device 100 for a mouse intervertebral disc degeneration model, which includes: a base plate 101 , a water tank 102 and a plurality of modeling cylinders 103 . The upper surface of the substrate 101 is provided with a modeling room 104 and a rest room 105 adjacent to the modeling room 104 for mice to move freely (refer to Figure 9 and Figure 10 ).

[0051] The molding equipment 100 is combined with the molding room 104 and the rest room 105, and the functions are integrated and the operation is convenient. The tops of the moulding chamber 104 and the rest room 105 have openings that can be manipulated to place objects inside.

[0052] refer to Figure 13 and Figure 14 , the molding cylinder 103 has a cylindrical structure, which is made of plexiglass material and has the function of light transmission. During modeling, provide the mice with adequate light. Of course, in other embod...

experiment example

[0075] Experimental group:

[0076] refer to figure 1 , 32 male 8-week-old C57BL / 6 mice were purchased from the Guangdong Provincial Medical Laboratory Animal Center, with an average weight of 23.78±1.37g. Experimental group: 16 mice were randomly selected, and the modeling equipment and method of the above-mentioned embodiment were used for modeling, as the experimental group; control group: the other 16 mice were used as the control group, except that the water tank was not filled with water, the rest were treated with the experimental group. The experimental mice were housed in the Guangdong Orthopaedic Research Institute, a standard clean-grade animal room with constant temperature / humidity.

[0077] Mice were randomly sacrificed at 6 weeks (POST 6W) and 10 weeks (POST 10W) ​​after modeling to evaluate the degree of degeneration of the intervertebral disc and facet joints. The obtained double feet were verified by imaging examination, histological staining and immunohist...

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Abstract

The invention discloses a model-making device, a model-making method and an application of a mouse intervertebral disc degeneration model. The molding equipment includes: one or more molding cylinders and a water tank for placing the molding cylinders, the position of the side wall of the molding cylinders close to the bottom wall or the bottom wall of the molding cylinders is provided with a plurality of water-permeable holes, and the water-permeable The hole communicates with the cavity inside the cylinder for placing the mouse. The modeling equipment can guide the water-avoiding habit of mice, make them maintain bipedal standing posture for a long time, increase the axial stress of mice, accelerate the process of lumbar disc degeneration, form a model of intervertebral disc degeneration, and provide a basis for the mechanobiology of intervertebral disc degeneration. Mechanistic studies of signal transduction provide effective mechanistic animal models.

Description

technical field [0001] The invention relates to the field of intervertebral disc degeneration research, in particular to a modeling device, a modeling method and an application for a mouse intervertebral disc degeneration model. Background technique [0002] Intervertebral disc degeneration is an important factor leading to low back pain, and animal models are an important basis for the study of intervertebral disc degeneration. For a long time, mice have been widely used in the study of intervertebral disc degeneration as an important experimental animal. Its advantages are as follows: 1. Low price and easy operation; 2. Short time period and high benefit; 3. Tissue probes and markers Fourth, the genome is easy to modify, and a variety of genetically modified mice can be created for related research [0003] At present, the methods of intervertebral disc modeling in mice mainly fall into the following categories: 1. Mechanically induced models, including pressure models an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 黄敏军敖翔张忠民王亮
Owner 南方医科大学第三附属医院(广东省骨科研究院)
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