Moulding device, moulding method and application of mouse intervertebral disc degeneration model

A technology of intervertebral disc and modeling, applied in the field of intervertebral disc degeneration research, can solve the problems of long period, poor simulation and high technical level

Active Publication Date: 2019-03-29
南方医科大学第三附属医院(广东省骨科研究院)
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. In the mechanical induction model, the mechanical compression model requires professional equipment and is poor in operability, and the stress site is the tail vertebra of the mouse, so the simulation is insufficient. On the one hand, the instability model induces intervertebral disc degeneration for a long time, which is not easy On the other hand, fibrosis of the surrounding tissues after surgery may re-stabilize the unstable spine, which is unreliable and has poor repeatability; second, the structural damage model is fast and effective, but its simulation is poor , the transient structural changes caused by trauma account for a large proportion of pathological development; three: natural induction, although the simulation is good, but the cycle is long, the study proves that the intervertebral disc of 18-month-old mice has obvious degenerative changes, which brings Higher cost; 4: Spontaneous model, fast and has a certain degree of simulation but requires high technical level, complicated experimental process and persistent interference factors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Moulding device, moulding method and application of mouse intervertebral disc degeneration model
  • Moulding device, moulding method and application of mouse intervertebral disc degeneration model
  • Moulding device, moulding method and application of mouse intervertebral disc degeneration model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] Please refer to Figure 9-Figure 12 , a modeling device 100 for a mouse intervertebral disc degeneration model provided in this embodiment, which includes: a base plate 101 , a water tank 102 and a plurality of modeling cylinders 103 . The upper surface of the base plate 101 is provided with a modeling chamber 104 and a lounge 105 adjacent to the modeling chamber 104 for mice to move freely (refer to Figure 9 and Figure 10 ).

[0051] The modeling equipment 100 is combined with a modeling room 104 and a rest room 105 to integrate functions and facilitate operation. The tops of the modeling room 104 and the rest room 105 have openings, which can be operated to place objects into them.

[0052] refer to Figure 13 and Figure 14 , the molding cylinder 103 has a cylindrical structure, which is made of plexiglass material and has a light-transmitting effect. During modeling, provide mice with sufficient light. Of course, in other embodiments, it can also be made of...

experiment example

[0075] Experimental group:

[0076] refer to figure 1 32 male 8-week-old C57BL / 6 mice were purchased from Guangdong Medical Experimental Animal Center, with an average body weight of 23.78±1.37g. Experimental group: 16 mice were randomly selected, and modeled using the modeling equipment and method of the above-mentioned embodiment, as the experimental group; Control group: The other 16 mice were used as the control group, except that the water tank was not filled with water, and the rest were treated with the experimental group. The experimental mice were kept in a standard clean animal room with constant temperature / humidity in Guangdong Academy of Orthopedics.

[0077] Mice were sacrificed randomly at 6 weeks (POST 6W) and 10 weeks (POST 10W) ​​after modeling to evaluate the degree of degeneration of intervertebral discs and facet joints. Accelerated degeneration of spinal motor units in a standing mouse model of disc degeneration. The result is as follows:

[0078] (1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a moulding device, moulding method and application of a mouse intervertebral disc degeneration model. The moulding device includes one or more moulding barrels and a water tankfor placing the moulding barrel. A position, close to the bottom wall, of the side wall of the moulding barrel or the bottom wall of the moulding barrel is provided with a plurality of water permeating holes, and the water permeating holes communicate with a moulding chamber used for placing mice and located inside the moulding barrel. The moulding device can guide a water avoiding habit of the mice, allows the mice to maintain a bipedal standing posture for a long time, increases the axial stress of the mice, accelerates the process of lumbar intervertebral disc degeneration, forms the intervertebral disc degeneration model, and provides an effective mechanical animal model for the study on the mechanism of mechanical and biological signal transduction of intervertebral disc degeneration.

Description

technical field [0001] The invention relates to the field of intervertebral disc degeneration research, in particular to a modeling device, modeling method and application of a mouse intervertebral disc degeneration model. Background technique [0002] Intervertebral disc degeneration is an important factor leading to low back pain, and animal models are an important basis for studying intervertebral disc degeneration. For a long time, mice, as important experimental animals, have been widely used in the related research of intervertebral disc degeneration. Its advantages are as follows: 1. Low price and easy operation; Fourth, the genome is easy to modify, and can create a variety of genetically modified mice for related research [0003] At present, the intervertebral disc modeling methods for mice mainly fall into the following categories: 1. Mechanical induction models, including pressure models and instability models; 2. Injury models, that is, damage to the annulus fi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 黄敏军敖翔张忠民王亮
Owner 南方医科大学第三附属医院(广东省骨科研究院)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap