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A Molecular Marker Related to Sheep Feed Conversion Rate and Its Application

A technology of feed conversion rate and molecular markers, which is applied in the field of molecular marker preparation, can solve the problems of less systematic analysis, and achieve the effects of simple operation, great practical application value, and low cost

Active Publication Date: 2022-03-25
甘肃润牧生物工程有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the indicators are analyzed according to the animal phenotype, and there are few systematic analyzes on the feed conversion rate of sheep at the genetic level

Method used

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  • A Molecular Marker Related to Sheep Feed Conversion Rate and Its Application
  • A Molecular Marker Related to Sheep Feed Conversion Rate and Its Application
  • A Molecular Marker Related to Sheep Feed Conversion Rate and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Amplification of ZEB2 gene

[0034] (1) Primer design

[0035] Using the sheep ZEB2 gene DNA (GenBank accession number: NC_019459.2) as the template, a pair of primers M-F and M-R were designed using Primer5.0 software. The primer sequences are as follows

[0036] ZEB2:

[0037] M-F: 5'-AGCAGCAACAACAGCAAT-3' (SEQ ID NO: 2),

[0038] M-R: 5'-GAATGACAACCGACTCCA-3' (SEQ ID NO: 3).

[0039] (2) Amplification and sequencing of PCR products

[0040] Using sheep genome as a template, M-F and M-R primers were used for PCR amplification, and the purified PCR product was connected with pMD-18T vector (purchased from Bao Bioengineering Dalian Co., Ltd.) in a water bath at 4 °C overnight; ~120μL of competent cells were placed in a 1.5mL Ependorff tube, 5μL of the ligation product was added and mixed, placed on ice for 30min, heat-shocked at 42°C for 90s, ice bathed for 3-4min, and 400μL of antibiotic-free LB liquid medium was added. Incubate with shaking at 37°C for ...

Embodiment 2

[0043] Example 2. Establishment of PCR-RFLP detection method

[0044] (1) PCR amplification and sequence analysis

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Abstract

The invention provides a molecular marker related to sheep feed conversion rate, as well as a detection method and application of the molecular marker. In the present invention, by performing PCR amplification and sequence analysis on the sheep ZEB2 gene, it is found that there is a T / C polymorphic site in the 645th position of the amplified fragment, and PCR-RLPF is further used to analyze the polymorphic site of 137 Hu sheep. Points are detected and the least squares model is established, and genotype and feed conversion rate are carried out correlation analysis, and finally determined that the ZEB2 gene fragment amplified by the present invention can be used as a molecular marker related to sheep feed conversion rate, and the C allele is dominant allele. The molecular marker of the invention can be used for the selection of grain-saving sheep and the cultivation of new breeds of grain-saving high-quality mutton sheep, and provides genetic engineering means for the genetic improvement of sheep feed conversion rate, and has great practical application value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker preparation, and in particular relates to a ZEB2 gene fragment as a molecular marker affecting the conversion rate of sheep feed and its application. Background technique [0002] With the development of the economy, people's demand for meat is increasing, among which mutton is one of the most demanded ingredients. According to statistics, there are currently about 300 million sheep and goats in the country (Zhao Youzhang. Development Trends, Problems and Countermeasures of Sheep Raising Industry at Home and Abroad. Modern Animal Husbandry and Veterinary Medicine, 2015, (09): 63-68). my country has a large population but limited pastures , Most sheep are fed under house feeding conditions and are highly dependent on food. How to alleviate the contradiction between humans and animals for food to the greatest extent and improve the feed conversion rate of the flock has become more and more i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 王维民张德印张小雪李冲喇永富李国泽
Owner 甘肃润牧生物工程有限责任公司
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