Anatomical fixing device for sampling early embryo specimen of mouse
A technology for fixing devices and specimens, which is applied in sampling devices, veterinary instruments, and equipment for restraining animals, etc. It can solve problems such as inability to guarantee embryo embedding and slicing angles, poor fixation effect of embryo specimens, and indistinguishable orientation of embryos from head to tail. , achieve good specimen fixation effect, reduce operating time loss, and reduce the effect of damage rate
Active Publication Date: 2019-04-09
BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV +1
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Problems solved by technology
Another treatment method is to directly fix the embryo together with the surrounding uterine wall without dissecting the uterus. This method is likely to lead to: ①Due to the protection of the uterine wall, the fixative is not easy to penetrate, and the fixation effect of the embryo sample is not good; ②Early developmental stage The embryo is in the process of rotation. Due to the covering of the uterine wall, the direction of the embryo from the end to the end cannot be distinguished, and the angle of embryo embedding and sectioning cannot be guaranteed. Usually, dozens of specimens need to be repeated blindly to meet the needs, resulting in a great waste of time, energy and animals.
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[0034] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.
[0035] Such as figure 1 Shown, a kind of anatomical fixation device that is used for mouse early embryo sample sampling, comprises fixing frame, also includes fixing frame, ophthalmic tweezers 13, specimen orifice plate 15, watch glass base 12, watch glass 11, also be provided with will A visual magnification device 7 for amplifying and displaying the material collection process;
[0036] The fixed frame includes a fixed base 1, an adjustable height and horizontal adjustment frame 2, and the end of the adjustable frame 2 away from the fixed base 1 is provided with a cutting knife clamping part 8;
[0037] The cutting knife clamping portion 8 and the adjusting frame are respectively provided with a cla...
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The invention discloses an anatomical fixing device for sampling an early embryo specimen of a mouse, and relates to the technical field of medical experimental instruments. The device comprises a fixing frame, and further comprises a fixing frame, a pair of ophthalmic forceps, a specimen pore plate, a watch glass base and a watch glass, wherein a visual amplifying device for amplifying and displaying a sampling process is also provided, and a specimen pore plate cover is arranged on the specimen pore plate. According to the device, the early embryo specimen can be quickly, controllably and visually processed, the working efficiency of an operator is improved, and the operation time loss is reduced; the damage rate of the early embryo of the mouse is reduced in a process of processing theearly embryo specimen, and the early embryo of the mouse is saved; and a good specimen fixing effect is achieved.
Description
technical field [0001] The invention relates to the technical field of medical experimental equipment, in particular to an anatomical fixation device for taking early mouse embryo specimens. Background technique [0002] Mouse embryos are an important tool for scientific research such as embryonic development research and congenital malformation research. Early mouse embryos are extremely small and difficult to obtain from the uterus, especially for morphological studies based on paraffin specimens. However, the study of embryos at this stage is a key step in understanding mouse development. [0003] At present, there are two methods for the treatment of early mouse embryo paraffin specimens before fixation. One treatment method is to dissect out the embryos in the uterus and then fix them (quoted from "Mouse Embryo Operation Experimental Manual (Third Edition) 》), this method is likely to cause: ① Microdissection takes a long time, which is likely to cause protein or RNA d...
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Login to View More IPC IPC(8): G01N1/04A61D3/00
CPCA61D3/00G01N1/04
Inventor 师亮陈俊
Owner BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV