Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for increasing targeting force of NK cells and application of CCR5 gene

A technology of NK cells, 1. CCR5, applied in the biological field, can solve the problems of restricting NK cell therapy and weak directional movement of NK cells, and achieve strong tumor infiltration ability, good clinical transformation prospects, and the effect of inhibiting tumor growth.

Active Publication Date: 2019-04-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ability of traditionally applied NK cells to move toward tumor sites is weak, and only a small number of NK cells can infiltrate malignant tissues, which greatly limits NK cell therapy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for increasing targeting force of NK cells and application of CCR5 gene
  • Method for increasing targeting force of NK cells and application of CCR5 gene
  • Method for increasing targeting force of NK cells and application of CCR5 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0024] 1. Acquisition of CCR5 coding sequence

[0025] After the total RNA of human peripheral blood mononuclear cells was extracted with Trizol reagent (Invitrogen), it was reverse transcribed into cDNA. Then, PCR amplification obtains the coding sequence of CCR5 (as shown in SEQ ID NO.1), wherein upstream primer sequence: 5'- ggatcc atggattatcaagtgtcaag-3' (the underline is the BamHI restriction site), downstream primer sequence: 5'- gcggccgc caagccccacagatatttc-3' (the underline is the NotI restriction site).

[0026] 2. Construction of CCR5 lentiviral overexpression plasmid

[0027] The PCR product of lentiviral plasmid pCDH-EF1-MSC-T2A-conGFP or CCR5 coding sequence was double digested with BamHI and NotI, respectively. After...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the targeting force of NK cells and an application of CCR5 gene in enhancing the specific motor ability of the NK cells to tumors. The method can transfect the CCR5 gene into the NK cells, and the NK with high expression of CCR5 will have stronger specific exercise ability to tumors, and can better control the tumors.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for improving the targeting force of NK cells and the application of CCR5 gene. Background technique [0002] Natural killer cells (NK) are a group of immune cells with strong tumor killing ability and have good clinical application prospects. Clinical trials have proved that NK cell infusion therapy can effectively control tumors and improve patient survival. However, the ability of traditionally applied NK cells to move toward tumor sites is weak, and only a small number of NK cells can infiltrate malignant tissues, which greatly limits NK cell therapy. Chemokines and their receptors play an important role in the process of affecting the directional movement of immune cells to specific tissues, and high expression of specific chemokine receptors can enhance the specific tissue aggregation ability of NK cells. Studies have shown that the chemokine CCL5 is high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/867C12N7/00C12N5/10C12R1/93
CPCC12N5/0646C12N7/00C12N15/86C12N2510/00C12N2740/15043C12N2740/15021
Inventor 盛誉乔李峰
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV