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Protein chip used for detecting veterinary drug residues in pork, and preparation method thereof

A protein chip and veterinary drug residue technology, applied in the biological field, can solve the problems of being unable to be adopted on a large scale, high error probability, and slow detection speed, and achieve the effects of simplifying the pre-processing process, high accuracy of results, and reducing detection costs

Inactive Publication Date: 2019-04-12
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these six indicators can be measured, there are disadvantages such as slow detection speed and high detection cost.
Moreover, there are practical problems such as inconvenient operation and high error probability in the separate detection of the six indicators.
As the most authoritative HPLC (liquid chromatography) method for trace component detection, although the result is accurate and reliable, the cost is extremely high, the equipment is expensive, and it cannot be adopted on a large scale.
Whether pork contains antibiotics and whether it is safe to eat is a question that the public is eager to know. In order to speed up the detection, many companies, testing agencies, and government law enforcement agencies have to spend more manpower, material resources, and time for testing. CLEN, RAC, SAL, CAP, CPZ, AMC caused a lot of waste

Method used

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  • Protein chip used for detecting veterinary drug residues in pork, and preparation method thereof
  • Protein chip used for detecting veterinary drug residues in pork, and preparation method thereof
  • Protein chip used for detecting veterinary drug residues in pork, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0036] (1) Black slide pretreatment;

[0037] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0038] ② Soak black slides in 0.05% silane solution (medium: 25% ethanol) for 60 minutes;

[0039] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 180°C for 0.2h.

[0040] (2) Antigen solution spotting;

[0041] refer to figure 1 , using machine automation to spot CLEN antigen solution, RAC antigen solution, SAL antigen solution, CAP antigen solution, CPZ antigen solution, AMC antigen solution, the concentration of the antigen solution is 0.05mg / mL, and each point is 20nL, and the distribution of each antibody is as follows: figure 1 shown.

[0042] (3) closed process;

[0043] The spotted bla...

Embodiment 2

[0053] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0054] (1) Black slide pretreatment;

[0055] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 24 hours, and then wash it with purified water for 2 to 8 times;

[0056] ② Soak black slides in 0.5% silane solution (medium: 25% ethanol) for 30 minutes;

[0057] ③Purge the soaked black glass slides with nitrogen gas, put them in an oven, and bake them at 140°C for 0.5h.

[0058] (2) Antigen solution spotting;

[0059] refer to figure 1 , using machine automation to spot CLEN antigen solution, RAC antigen solution, SAL antigen solution, CAP antigen solution, CPZ antigen solution, AMC antigen solution, the concentration of the antigen solution is 0.05mg / mL, and each point is 20nL, and the distribution of each antibody is as follows: figure 1 shown.

[0060] (3) closed process;

[0061] The spotted blac...

Embodiment 3

[0071] A protein chip for the detection of veterinary drug residues, the preparation method of the protein chip comprises the following steps:

[0072] (1) Black slide pretreatment;

[0073] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 20 hours, and then wash it with purified water for 2 to 8 times;

[0074] ② Soak black slides in 1% silane solution (medium: 25% ethanol) for 20 minutes;

[0075] ③Purge the soaked black glass slides with nitrogen gas, put them into an oven, and bake them at 100°C for 0.6h.

[0076] (2) Antigen solution spotting;

[0077] refer to figure 1 , using machine automation to spot CLEN antigen solution, RAC antigen solution, SAL antigen solution, CAP antigen solution, CPZ antigen solution, AMC antigen solution, the concentration of the antigen solution is 0.05mg / mL, and each point is 20nL, and the distribution of each antibody is as follows: figure 1 shown.

[0078] (3) closed process;

[0079] The spotted blac...

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PUM

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Abstract

The invention discloses a protein chip used for detecting veterinary drug residues in pork. The preparation method for the protein chip comprises the following steps that: (1) preprocessing a black slide; (2) carrying out antigen solution sample application; and (3) carrying out a sealing technology to prepare the protein chip. The protein chip is used for detecting the veterinary drug residues inthe pork, and the original protein chip technology of the company is used for realizing combined detection to effectively improve detection efficiency and lower detection cost. The inventor developsa protein chip diagnosis kit which contains six indexes, including CLEN (clenbuterol hydrochloride), RAC (ractopamine), SAL (salbutamol), CAP (chloramphenicol), CPZ (chlorpromazine) and AMC (amoxicillin), and the kit has the advantages of being quick, efficient, low in cost and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein chip for detecting veterinary drug residues and a preparation method thereof. Background technique [0002] Veterinary drug residues refer to the accumulation or retention of the original drug or its metabolites in the body of livestock or poultry or in the product after drug administration. The correct and reasonable use of veterinary drugs has brought high benefits to the breeding industry, but there are always some people who blindly pursue economic interests and illegally use, abuse, add or inject veterinary drugs or harmful substances in all aspects of the livestock and poultry industry. The residues and harmful substances of veterinary drugs seriously affect the safety of animal food and the sustainable development of animal husbandry, and more seriously, they directly endanger human health and life safety, and are extremely harmful to human health. Therefore, animal...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/552G01N33/531
CPCG01N33/531G01N33/552G01N33/6803
Inventor 邓仲锦泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
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