Three-dimensional microscopic imaging method and system based on a light field microscopic system

Abstract

The invention discloses a three-dimensional microscopic imaging method and system based on a light field microscopic system. The method comprises the following steps: S1, in an optical system, obtaining a first point spread function of a three-dimensional sample from an object plane to a main camera sensor plane and a second point spread function from the object plane to a secondary camera sensorplane, and generating a first forward projection matrix and a second forward projection matrix according to the first point spread function and the second point spread function; s2, acquiring a lightfield intensity image shot by a primary camera and a high-resolution intensity image shot by a secondary camera of the three-dimensional sample in an optical system; and S3, performing three-dimensional reconstruction on the light field intensity image, the first forward projection matrix, the high-resolution intensity image and the second forward projection matrix through a preset algorithm to generate a three-dimensional reconstruction result of the three-dimensional sample. According to the method, the focal plane reconstruction signal-to-noise ratio is enhanced under the same iteration frequency by adding one path of acquisition light path, and the reconstruction effect of light field microscopic imaging is greatly improved.

Description

technical field [0001] The invention relates to the technical field of computational imaging, in particular to a three-dimensional microscopic imaging method and system based on a light field microscopic system. Background technique [0002] Usually, the microscopic samples that need to be observed are often three-dimensional. In traditional microscopic techniques, only one depth of the sample can be imaged two-dimensionally, and the sample slice can be observed, but the three-dimensional structure of the sample cannot be observed, which cannot meet the needs of some biological fields. If it is necessary to collect data of three-dimensional samples, it is necessary to collect multiple images of different depths through longitudinal axis scanning and other methods, and then perform axial splicing. This method is relatively slow. In the case of high dynamics, the scanning speed of the vertical axis Below the dynamic change rate of biological structures, it is difficult to ima...

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Problems solved by technology

However, this method is limited by the sparse spatial distribution at the imaging focal plane, making it difficult to improve the reconstruction resolution at the focal plane

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