A kind of production method of spinosad

A production method, spinosad technology, applied in the field of spinosad production, can solve the problems of low spinosad yield and purity, large solvent consumption, long extraction time, etc., and achieve simple and easy fermentation and separation and purification methods , improve the purity, improve the effect of cell activity

Active Publication Date: 2020-01-17
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the extraction process of this invention, the Soxhlet extraction usually takes a long time and the yield is low, and the recrystallization process will consume part of n-pentane, n-hexane, cyclohexane or petroleum ether, etc., and the solvent consumption is large, which will affect the environmental impact
[0007] So far, the production of spinosyn has various problems such as complicated process, high cost, and environmental pollution, and the yield and purity of the prepared spinosad are usually low. Therefore, the production process of spinosyn needs to be further optimized. Then increase the yield and purity of spinosad, simplify the process, reduce cost and solvent consumption, and avoid the occurrence of environmental pollution problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A production method of spinosyn, comprising the following steps:

[0050] (1) Fermentation:

[0051] S1: slant culture: culture Saccharopolyspora spinosa on the slant medium to make spore suspension;

[0052] The components of the slant medium are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 3g / L, MgSO 4 ·7H 2 O 0.2g / L and agar powder 16g / L;

[0053] Slope culture conditions: culture temperature is 28°C, rotation speed is 150r / min, culture cycle is 10 days, pH is 6.5;

[0054] S2: Seed culture: the spore suspension obtained in step S1 is transferred to the seed activation medium with a volume ratio of 1% inoculum to obtain a seed liquid;

[0055] Seed activation medium components are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 4g / L, casein peptone 25g / L, MgSO 4 ·7H 2 O0.2g / L and K 2 HPO 4 1.3g / L;

[0056] Seed culture conditions: the culture temperature is 30°C,...

Embodiment 2

[0067] A production method of spinosyn, comprising the following steps:

[0068] (1) Fermentation:

[0069] S1: slant culture: culture Saccharopolyspora spinosa on the slant medium to make spore suspension;

[0070] The components of the slant medium are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 3g / L, MgSO 4 ·7H 2 O 0.2g / L and agar powder 16g / L;

[0071] Slope culture conditions: culture temperature is 28°C, rotation speed is 150r / min, culture cycle is 10 days, pH is 6.5;

[0072] S2: Seed culture: the spore suspension obtained in step S1 is transferred to the seed activation medium with a volume ratio of 1% inoculum to obtain a seed liquid;

[0073] Seed activation medium components are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 4g / L, casein peptone 25g / L, MgSO 4 ·7H 2 O0.2g / L and K 2 HPO 4 1.3g / L;

[0074] Seed culture conditions: the culture temperature is 30°C,...

Embodiment 3

[0085] A production method of spinosyn, comprising the following steps:

[0086] (1) Fermentation:

[0087] S1: slant culture: culture Saccharopolyspora spinosa on the slant medium to make spore suspension;

[0088] The components of the slant medium are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 3g / L, MgSO 4 ·7H 2 O 0.2g / L and agar powder 16g / L;

[0089] Slope culture conditions: culture temperature is 28°C, rotation speed is 150r / min, culture cycle is 10 days, pH is 6.5;

[0090] S2: Seed culture: the spore suspension obtained in step S1 is transferred to the seed activation medium with a volume ratio of 1% inoculum to obtain a seed liquid;

[0091] Seed activation medium components are calculated by mass concentration, including: glucose 10g / L, soluble starch 5g / L, yeast extract 4g / L, casein peptone 25g / L, MgSO 4 ·7H 2 O0.2g / L and K 2 HPO 4 1.3g / L;

[0092] Seed culture conditions: the culture temperature is 30°C,...

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PUM

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Abstract

The invention provides a method for producing spinosad, and belongs to the technical field of fermentation engineering, the method optimizes the fermentation, separation and purification processes ofthe spinosad and comprises the following steps of: adding lecithin into a fermentation culture solution, optimizing culture medium components and culture conditions, supplementing materials in the fermentation process and improving the extraction process in the separation and purification process, so that the activity of the polysporaspinosa is increased, the yield and purity of the polysporaspinosa are improved, and the method has the advantages of small environmental pollution, low cost, high efficiency, simple process and the like.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a production method of spinosyns. Background technique [0002] Spinosyn is a kind of macrolide pollution-free and high-efficiency biopesticide extracted from the fermentation broth of Polyspora spinosa. As a broad-spectrum biopesticide, spinosyn has the advantages of high efficiency, low toxicity, low residue, easy degradation and harmlessness to non-target animals, and has no carcinogenic, teratogenic or mutagenic effects on mammals. [0003] At present, the complex fermentation process, low efficiency and high cost are the main problems restricting the large-scale production of spinosyns, and the optimization of the medium and the transformation of strains are the main methods to increase the fermentation yield and reduce the fermentation cost. Usually, the technical requirements for strain transformation are relatively high and difficult to imple...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C07H17/08C07H1/06C12R1/645
CPCC07H1/06C07H17/08C12P19/62
Inventor 常俊义陈峰钟迎东叶朋侯争平
Owner 内蒙古拜克生物有限公司
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