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Identification method and application of eggs of Haemonchus contortus

A technology of haemonchus contortus and eggs, which is applied in the field of identification of haemonchus contortus eggs, can solve the problems of distinguishing eggs, not finding eggs, distinguishing, etc., achieving low cost and easy operation Simplicity, the effect of alleviating detection difficulties

Active Publication Date: 2021-11-12
SICHUAN ANIMAL SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because most of the eggs have very little difference in appearance, it is difficult to distinguish the eggs only by taking pictures under a microscope or their size. Distinctive traits that distinguish it from other worm eggs

Method used

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  • Identification method and application of eggs of Haemonchus contortus
  • Identification method and application of eggs of Haemonchus contortus
  • Identification method and application of eggs of Haemonchus contortus

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Embodiment 1

[0133] The present embodiment provides a method for identifying the eggs of Haemonchus contortus, comprising the steps of:

[0134] 1. Collecting nematode eggs: collecting eggs by sedimentation method includes the following steps. Collect fresh fecal samples from cattle and sheep rectally. After crushing, wash and filter with an 80-mesh filter, discard the feces, and then filter the filtrate with a filter for 2~ 3 times, stand still for 20-30 minutes, pour off the supernatant; settle with clean water for 20-30 minutes, repeat this way 2-3 times to get eggs;

[0135] 2. Cultivate and observe the number of nematode eggs containing larvae (embryo like tadpole or snake-like) and air chamber eggs: place the eggs in normal saline at about 23°C for 4 days, and examine them under a microscope Under 400 times, observe the number of eggs containing larvae and the number of eggs containing air cells;

[0136] 3. Observe the appearance of nematode eggs (length, width and aspect ratio). ...

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Abstract

The invention provides a method for identifying eggs of Haemonchus contortus and an application thereof, and relates to the field of nematode identification. The method for identifying the eggs of Haemonchus contortus of the present invention comprises the following steps: collecting the eggs of the nematode, wherein the eggs of the nematode include: eggs of Haemonchus contortus, eggs of Osteria nematodes, and eggs of Chabertes nematodes Eggs and esophageal stoma nematode eggs; by observing the number of larvae eggs contained in each nematode egg after culture, the air chamber and appearance of nematode eggs and whether the nematode eggs can be combined with peanut lectin, the twirling Haemonchus ova are distinguished from ova of Osteria, Chabertia, and esophagostoma. The method is simple in operation and low in cost, and can accurately and efficiently distinguish the eggs of Haemonchus contortus from the other three kinds of nematodes, and can alleviate the difficulty in detecting Haemonchus contortus in animals, high detection cost, complicated operation and troublesome twirling. The technical problem that Haemonchus eggs are not easy to distinguish from other nematode eggs.

Description

technical field [0001] The invention relates to the field of nematode identification, in particular to a method for identifying eggs of Haemonchus contortus and its application. Background technique [0002] Common nematodes in the digestive tract of cattle and sheep include Haemonchus contortus, Osteria, Chabertus and esophageal stoma, among which Haemonchus contortus is the most pathogenic and most harmful to cattle and sheep. A parasitic nematode, this nematode mainly lives in the abomasum of cattle and sheep, occasionally in the small intestine, and feeds on the blood of the host. Currently popular methods for detecting parasitic diseases include egg detection methods, immunological detection methods and nucleic acid detection methods. These detection methods are very important for the differential diagnosis of Haemonchus contortus disease in cattle and sheep. Among them, the detection of eggs is intuitive, accurate, easy to operate, and can reflect the infection statu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N1/30A01K67/033
CPCA01K67/033G01N1/30G01N21/6458
Inventor 叶勇刚廖党金康润敏魏勇林毅谢晶叶建强戴卓建于吉峰曹冶李兴玉肖璐潘梦
Owner SICHUAN ANIMAL SCI ACAD
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