Method for obtaining first-dimension chromatography peak in comprehensive two-dimensional chromatography through two modulation peaks

A two-dimensional chromatography and chromatographic peak technology, which is applied in the derivation field of the first-dimensional chromatographic peak, chromatographic peak retention time, and achieves the effect of high accuracy and small error

Active Publication Date: 2019-04-26
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] The technical problem to be solved by the present invention is to overcome the limitation that more than three modulation peaks are required in the derivation process of the first-dimensional chromatographic peak in the existing comprehensive two-dimensional chromatogram, and to provide a development method that can use two modulation peaks to accurately derive the first The method of two-dimensional chromatographic peaks is applied to the calculation of first-dimensional chromatographic peaks in comprehensive two-dimensional chromatography, especially the calculation of peak retention time and retention index, to assist the qualitative analysis of unknowns

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  • Method for obtaining first-dimension chromatography peak in comprehensive two-dimensional chromatography through two modulation peaks
  • Method for obtaining first-dimension chromatography peak in comprehensive two-dimensional chromatography through two modulation peaks
  • Method for obtaining first-dimension chromatography peak in comprehensive two-dimensional chromatography through two modulation peaks

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Embodiment 1

[0041] The comprehensive two-dimensional gas chromatographic conditions used in the method for deriving the first-dimensional chromatographic peak in comprehensive two-dimensional chromatography based on two modulated chromatographic peaks described in this example are as follows: Agilent 7890A gas chromatograph, and the detector is Agilent 7200QTOFMS , the comprehensive two-dimensional chromatographic modulator is LMCS (Chromatography Concepts Ltd, Doncaster, Australia). With helium as the carrier gas, the flow rate is 1.2mL / min. The initial column temperature of the gas chromatograph was 40°C and maintained for 2 minutes, then raised to 240°C at 5°C / min and maintained for 20 minutes. SUPELCOWAX 10 capillary column (specification: 30m′0.25mm I.D.′0.25μm; Supelco, Bellefonte, PA) was used as the first dimension column, and Rxi-5SilMS capillary column (specification: 1.0m′0.1mm I.D.′0.1μm ; Restek Corp., Bellefonte, PA) is a second-dimensional chromatographic column, and the t...

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Abstract

The invention discloses a method for obtaining a first-dimension chromatography peak in comprehensive two-dimensional chromatography through two modulation peaks. The method comprises the following steps of 1, preliminarily setting 1 sigma=PM / 3; 2, setting the area of a first-dimension peak as the sum of areas of two modulation peaks; 3, combining the formula 1 and the formula 2, and obtaining thebest 1tR value under the set 1 sigma condition according to a nonlinear planning method; 4, judging whether a result derived in step 3 is correct or not, and according to the judgment result, executing follow-up steps. The method has the advantages that the first-dimension chromatography peak in the comprehensive two-dimensional chromatography can be derived by means of two modulation peaks, andthe method is particularly applicable to derivation of the first-dimension chromatography peak in the comprehensive two-dimensional chromatography obtained under the undersampling condition; the method is high in accuracy, and the error of retention time obtained by calculation is small.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to chromatography, more specifically to a method for deriving first-dimensional chromatographic peaks in comprehensive two-dimensional chromatographic methods, especially the retention time of chromatographic peaks, so as to assist the qualitative analysis of unknowns. Background technique [0002] In chromatography, in order to improve the accuracy of qualitative analysis of unknowns, it is often necessary to determine the chromatographic retention time of unknowns. For comprehensive two-dimensional chromatography, the first and second dimension chromatographic retention times can be determined separately. For the retention time of a compound in the first-dimensional chromatogram in the comprehensive two-dimensional chromatogram, it can be obtained from the following three methods: [0003] 1. Change the comprehensive two-dimensional chromatography to ordinary one-dimensional chro...

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Application Information

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IPC IPC(8): G01N30/86
CPCG01N30/8634G01N30/8679
Inventor 江明
Owner HUAZHONG UNIV OF SCI & TECH
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