Method for inducing male sterility of temperature-sensitive dual-purpose line of high-winterness cabbage type winter rape

Abstract

The invention belongs to the field of crop breeding and relates to a method for inducing male sterility of a temperature-sensitive dual-purpose line of a high-winterness cabbage type winter rape. Themethod includes the steps: 1) in each mid-February, germinating and springing PK3-12S seeds in a lab; 2) planting the PK3-12S seeds treated in the step 1) in a Gansu Huining ecological region or a large field in a region at the latitude as same as that of Gansu Huining in mid-April, and managing the field via a process the same as a local management method of local cabbage type winter rapes; 3) when the PK3-12S seeds in the Gansu Huining ecological region or the large field in the region at the latitude as same as that of Gansu Huining blossom in late June, identifying fertility of the PK3-12Sat a flowering stage via a process of observing anther representation and judging pollen activity under the pollen activity judging standard of dyeing with I2-KI solution; 4) identifying fruiting capacity. Pollen dyed in blue is active pollen, and lightly-dyed or non-stained pollen is inactive abortive pollen. The method can reduce breeding difficulty and facilitate implementation.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a breeding induction method for winter rapeseed, in particular to a method for inducing male sterility of a temperature-sensitive dual-purpose line of winter rapeseed with strong winter character. Background technique [0002] The temperature-sensitive male sterile line PK3-12S of Chinese cabbage type winter rapeseed with strong winter sex is a special type of male sterile line whose fertility is regulated by external environmental conditions. The temperature in the external environment acts as a factor affecting fertility and directly affects With the continuation of the temperature influence time, the growth of pollen grains will not be sufficient in the early stage, and the anthers will not crack in the later stage, or the fertilization process will be directly affected, resulting in PK3-12S being fertile at low temperature and sterile at high temperature. The cabbage-type temperatu...

AI Technical Summary

Problems solved by technology

The cabbage-type temperature-sensitive male sterile line PK3-12S with strong winter sex cannot undergo vernalization when it is sown in spring; when it is sown in autumn and turns green and blooms in the next year, the external temperature is low, and it is fertile, which makes the seed production of the Chinese cabbage-type temperature-sensitive male sterile line PK3-12S The difficulty has been greatly increased

Images