Extracting method for soybean oligosaccharides

The technology of soybean oligosaccharide and extraction method is applied in the field of extraction of soybean oligosaccharide, which can solve the problems of difficult dissolution of soybean oligosaccharide, inability to separate soybean oligosaccharide, low extraction rate, etc., and achieves easy leaching and operation. The effect of short time and simple extraction process

Inactive Publication Date: 2019-05-10
安徽博悦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many extraction methods of soybean oligosaccharides, but the soybean oligosaccharides of most methods are not easy to dissolve, the extraction rate is not high, and the purity is low, so it is impossible to separate soybean oligosaccharides into stachyose, raffinose and sucrose, and cannot satisfy people's demands

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  • Extracting method for soybean oligosaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:

[0046] 1. Pretreatment: After the soybeans are crushed, extruded and extruded to obtain extruded soybeans, the extruded extruded and water are mixed, stirred and mixed evenly at a temperature of 45°C in an ultrasonic environment, and then enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;

[0047] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;

[0048] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the water phase mixture. The preci...

Embodiment 2

[0055] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:

[0056] 1. Pretreatment: crush the soybeans and perform extrusion and puffing treatment to obtain extruded soybeans. After mixing the extruded puffed substances with water, stir and mix them evenly at a temperature of 50°C in an ultrasonic environment, and then perform enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;

[0057] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;

[0058] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the ...

Embodiment 3

[0065] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:

[0066] 1. Pretreatment: crush the soybeans and perform extrusion processing to obtain extruded soybeans. After mixing the extruded extruded materials with water, stir and mix them evenly at a temperature of 55°C in an ultrasonic environment, and then perform enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;

[0067] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;

[0068] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the water phas...

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Abstract

The invention discloses an extracting method for soybean oligosaccharides. The method comprises the following steps of preprocessing, ultrasonic-microwave synergic processing, acid precipitate processing, digestion processing, decoloring, salt removal, concentration drying, MPLC separation and secondary MPLC separation. According to the extracting method for soybean oligosaccharides, dissolution of oligosaccharide is accelerated by means of the ultrasonic effect, meanwhile, the temperature is controlled within the proper range, the soybean cell wall is softened, the soybean structure is loosened, molecular movement is violent, the soybean oligosaccharides are more likely to be leached, the soybean oligosaccharides extracting rate is increased, by adopting ultrasonic-microwave synergic processing, ultrasonic vibration mass transfer and microwave energy transfer are combined, soybean oligosaccharides extraction is facilitated, oligosaccharides are separated through the MPLC, crude soybean oligosaccharides can be effectively separated into stachyose monomers, raffinose monomers and saccharose monomers, and the monomers of oligosaccharides can be applied separately.

Description

technical field [0001] The invention relates to the field of soybean product processing, in particular to a method for extracting soybean oligosaccharides. Background technique [0002] Soy oligosaccharides are the general term for soluble carbohydrates contained in soybeans. The main components are stachyose, raffinose and sucrose, and their dry weight contents in soybean oligosaccharides are 24%, 8%, and 39% respectively . The main functional ingredients raffinose and stachyose have the functions of laxative and intestinal cleansing, promoting the reproduction of bifidobacteria in the intestine, and lowering serum cholesterol. They can also be used to reduce water activity, inhibit microbial reproduction, and achieve freshness and moisturizing effects. Effect. Sucrose in soybean oligosaccharides can replace part of sucrose as a low-calorie sweetener. At present, soybean oligosaccharides are used as functional factors or food additives to produce various oral liquids, pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H3/06C07H3/04C08B37/00
Inventor 王其初王其平梁彥贾虎
Owner 安徽博悦生物科技有限公司
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