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Detection accuracy identifying method, detection accuracy identifying device, and detection accuracy identifying program

An identification method and accuracy technology, applied in the field of detection accuracy identification procedures, can solve the problem of not proposing calculation of LOD and so on

Pending Publication Date: 2019-05-14
RICOH KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no method has been proposed to calculate the LOD using this method

Method used

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  • Detection accuracy identifying method, detection accuracy identifying device, and detection accuracy identifying program
  • Detection accuracy identifying method, detection accuracy identifying device, and detection accuracy identifying program
  • Detection accuracy identifying method, detection accuracy identifying device, and detection accuracy identifying program

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0221] The test device preparation method preferably includes a cell suspension preparation step: preparing a cell suspension containing a plurality of cells including a specific nucleic acid and a solvent; a droplet landing step: discharging the cell suspension in the form of droplets so that the droplets successively land on the plate in the well; the cell number counting step: counting the number of cells contained in the droplet with a sensor after the droplet is discharged and before the droplet lands in the well; the nucleic acid extraction step: extracting nucleic acid from the cells in the well; and detecting A probability calculation step, and further includes other steps as required.

[0222]

[0223] The cell suspension preparation step is a step of preparing a cell suspension containing a plurality of cells including a specific nucleic acid and a solvent.

[0224] Solvent means the liquid used to disperse cells.

[0225] The suspension in the cell suspension mea...

Embodiment

[0422] Hereinafter, the present disclosure will be described by way of examples. The present disclosure should not be construed as being limited to these examples.

[0423]

[0424] The test device was prepared in the following manner.

[0425] -Recombinant Yeast-

[0426] For the preparation of recombinants, budding yeast YIL015W BY4741 (available from ATCC, ATCC4001408) was used as a carrier cell for one copy of the specific nucleic acid sequence. The specific nucleic acid sequence is a dense nucleic acid sample DNA600-G (available from National Institute of Advanced Industrial Science and Technology, NMIJ CRM6205-a). In the form of a plasmid prepared by arranging a specific nucleic acid sequence in tandem with URA3 (selectable marker), one copy of the specific nucleic acid sequence was introduced into yeast genomic DNA by homologous recombination, targeting the BAR1 region of the vector cell, thereby producing Genetic recombinant yeast.

[0427] -Culture and Cell Cycl...

Embodiment approach

[0460] Embodiments of the present disclosure are, for example, as follows:

[0461] The detection accuracy identification method for identifying the detection accuracy of the detection target nucleic acid, the detection accuracy identification method includes

[0462] The detection accuracy capability of the test target nucleic acid is identified using a standard material comprising a known number of nucleic acid molecules and based on the probability of detecting the known number of nucleic acid molecules.

[0463] According to the identification method of detection accuracy ,

[0464] Wherein identifying includes identifying the detection accuracy capability of the test target nucleic acid based on the probability and probability distribution of detecting a known number of nucleic acid molecules.

[0465] According to the detection accuracy identification method described in ,

[0466] where the probability distribution is a Poisson distribution.

[0467] According to ...

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Abstract

The invention relates to a detection accuracy identifying method, a detection accuracy identifying device, and a detection accuracy identifying program. Provided is a detection accuracy identifying method for identifying a detection accuracy for detecting a testing target nucleic acid, the method including identifying a detection accuracy ability of the testing target nucleic acid, using a standard substance containing a known number of nucleic acid molecule and based on a probability at which the known number of nucleic acid molecule is detected.

Description

field of invention [0001] The present disclosure relates to a detection accuracy identification method, a detection accuracy identification device, and a detection accuracy identification program. Background of the invention [0002] In recent years, increased sensitivity of analytical techniques has enabled measurement of measurement targets in units of molecular quantities, and industrial applications of gene detection techniques that detect trace amounts of nucleic acids have been required for food, environmental auditing, and healthcare. In particular, the detection of pathogens or unapproved genetically modified foods is usually to confirm the absence in the sample and requires a high level of detection accuracy. [0003] For example, based on the technical characteristics of nucleic acid detection methods using nucleic acid amplification represented by polymerase chain reaction (PCR) commonly used in the field of molecular biology research, it is said that even in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/81
CPCC12Q1/6851C12Q2537/165C12Q2545/113C12Q2561/113C12Q2563/159C12Q1/6844C12Q2600/166
Inventor 濑尾学和泉贤川岛优大
Owner RICOH KK
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