Human adipose preserving fluid and preparing method thereof

Abstract

The invention discloses human adipose preserving fluid and a preparing method thereof. Each 1000 ml of human adipose preserving fluid contains 50-70 g of low molecular dextran-40, 50-60 mmol of NaCl,20-30 mmol of KCl, 4-8 mmol of MgSO4, 40-60 mmol of cane sugar, 0.1-0.2 g of penicillin G sodium salt, 0.1-0.2 g of amphotericin, 0.07-0.13 g of gentamicin, 3-5 mmol of reduced glutathione, 4-8 mmol of adenosine and the balance deionized water. The preserving fluid has the pH value of 7.33-7.53. All the components are mixed and dissolved in the deionized water, subpackaged and sealed, and then preserved at 0-4 DEG C. The preserving fluid has a remarkable effect of reducing the adipose cell death rate and keeping the activity of mesenchymal stem cells in fat when used for preserving adipose tissue. In addition, bacteria can be effectively restrained, and the further clinical and scientific research application can be easily conducted on the fat.

Description

Technical field [0001] The invention relates to the field of preservation liquids, in particular to a human adipose tissue preservation liquid and a preparation method thereof. Background technique [0002] With the development of medicine, the role of adipose tissue has been continuously discovered. In addition to storing energy, adipose tissue also has an active endocrine function, can secrete a variety of hormones, and participate in the regulation of hormone levels. The biological role of mesenchymal stem cells has also been continuously discovered in scientific research and clinical applications. . In addition, in plastic medical technology, adipose tissue has also been found to have a good tissue replacement function and has a good market application prospect. Therefore, the preservation technology of adipose tissue is particularly important. [0003] At present, there is no specific preparation for preserving the fat group, and the preserving liquid prepared temporarily c...

AI Technical Summary

Problems solved by technology

[0003] At present, there is no specific preparation for the preservation of adipose tissue. Temporarily prepared preservation solutions often cannot preserve adipose tissue on a large scale for a long time, and the price and cost are high, and the effect of inhibiting bacteria is not ideal, which is not conducive to the preservation of fat.