Method for preparing collagen tripeptide by mixing water-soluble collagen with antioxidant

An anti-oxidant, water-soluble technology, applied in the field of preparation of tripeptide collagen, can solve the problem of short shelf life

Inactive Publication Date: 2019-05-28
孔祥圣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of tissue engineering, it is widely used in tissue culture, medical materials and other fields. The common type I tripeptide collagen is water-insoluble collagen. The common water-soluble collagen in the market is actually the degradation of type I collagen into polypeptides. The formed collagen peptides, undenatured water-soluble collagen that is completely different from type I collagen are also extracted from fetal bovine and calf skin, but due to the intermolecular force generated by its special spatial structure, it is very easy to produce cross-linking However, the tripeptide collagen converted into water-insoluble has a very short shelf life, so that it cannot be widely used in experiments and production processes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Take 100 mg of commercially available water-soluble collagen solution, add it into 1000 ml of deionized water, stir and dissolve to form a collagen solution with a mass-volume concentration of 0.1%. 50g of L-ascorbic acid was dissolved in 1000ml deionized water to make an antioxidant solution with a mass volume ratio concentration of 5%. 8°C, change the dialyzed external fluid every 4 hours, dialyze for 72 hours, centrifuge the dialyzate at 5000rpm at 2-8°C for 10min, take the supernatant and freeze-dry it.

Embodiment 2

[0010] Take 500 mg of commercially available water-soluble collagen solution, add it into 1000 ml of deionized water, stir and dissolve to form a collagen solution with a mass-volume concentration of 0.5%. Dissolve 100g of anthocyanidin in 1000ml of deionized water to make an antioxidant solution with a mass-volume concentration of 10%. ℃, change the dialysis fluid every 4 hours, dialysis for 72 hours, the dialyzate was centrifuged at 5000rpm for 10min at 2-8℃, and the supernatant was freeze-dried to shape.

Embodiment 3

[0012] Take 200 mg of commercially available water-soluble collagen solution, add it into 1000 ml of deionized water, stir and dissolve to form a collagen solution with a mass-volume concentration of 0.2%. Dissolve 70g of tea tannin in 1000ml of deionized water to make an antioxidant solution with a concentration of 7% by mass to volume. 8°C, change the dialyzed external fluid every 4 hours, dialyze for 72 hours, centrifuge the dialyzate at 5000rpm at 2-8°C for 10min, take the supernatant and freeze-dry it.

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PUM

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Abstract

The invention discloses a method for preparing collagen tripeptide by mixing water-soluble collagen with an antioxidant, and belongs to the field of medical biomaterials. The invention mainly aims atsolving the problem that the existing collagen tripeptide is easy to cross-link and modify, which leads to water insolubility in the storage process. The prepared water-soluble collagen is formed by combining water-soluble collagen tripeptide with the corresponding antioxidant; the method for preparing the stable collagen tripeptide by mixing the water-soluble collagen with the antioxidant benefits the preservation and application of the water-soluble tripeptide collagen.

Description

technical field [0001] The invention relates to a method for preparing tripeptide collagen by mixing water-soluble collagen and antioxidant. Background technique [0002] Tripeptide collagen is one of the extracellular matrix components produced by multicellular animals. With the development of tissue engineering, it is widely used in tissue culture, medical materials and other fields. The common type I tripeptide collagen is water-insoluble collagen. The common water-soluble collagen in the market is actually the degradation of type I collagen into polypeptides. The formed collagen peptides, undenatured water-soluble collagen that is completely different from type I collagen are also extracted from fetal bovine and calf skin, but due to the intermolecular force generated by its special spatial structure, it is very easy to produce cross-linking However, the tripeptide collagen converted into water-insoluble has an extremely short shelf life, so that it cannot be widely use...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C07K14/78C07K5/08
Inventor孔祥圣
Owner孔祥圣