Detection method and detection reagent for transgenic-maize strain MON87427

Abstract

The invention discloses a detection method and a detection reagent for a transgenic-maize strain MON87427. The detection method includes simultaneously adding specific primers and probes for exogenousgenes and HMG genes of the maize strain MON87427 into a PCR reaction system, adding mineral oil, performing uniform mixing to obtain droplets, carrying out double ddPCR reaction in the droplets, reading each droplet amplification signal after reaction, and calculating the copy numbers of the exogenous genes and the HMG genes of the strain, wherein the probes of the exogenous genes and the HMG genes of the strain are marked with FAM and HEX respectively. The detection method has the advantages that the endogenous HMG genes and the strain trait primer probes, which are used cooperatively, are designed, a two-channel method is applied, the copy numbers of the two in maize genomes are respectively quantified, and the absolute content of the transgenic-maize strain MON87427 is calculated, so that the method is of great significance in studies on transgenic components of maize and in accurate and quantitative detection of the transgenic components in inbound and outbound maize products.

Description

technical field [0001] The application relates to the field of transgenic maize detection, in particular to a detection method and reagents of a transgenic maize line MON87427. Background technique [0002] With the rapid development of the biotechnology industry, the research and development speed and planting area of ​​genetically modified crops continue to increase globally. Promoting genetically modified technology has become an inevitable choice for future-oriented development strategies and to solve food shortages and ensure national food security. However, the safety of genetically modified organisms has also become a hot spot in the world. At present, all countries in the world have strengthened the management and testing of genetically modified organisms and their products for various purposes such as economy, health, and environmental protection. About 50 countries and regions, including China, have promulgated and implemented the "GMO labeling system" to label a...

AI Technical Summary

Problems solved by technology

However, digital PCR is still in its infancy in the research of genetically modified detection, especially in the standard level of my country and even countries around the world, which greatly restricts the application of digital PCR in actual detection.

[0005] Studies have shown that, according to the statistics of the planting area, about 35% of the corn in the world are genetically modified products; however, international and domestic specific qualitative or quantitative detection methods for genetically modified corn are quite lacking, especially for the quantitative detection of the genetically modified corn line MON87427. There are relevant studies or reports

Images