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Rapid propagation method of Lepisorus ussuriensis

A Ussuri and fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult rooting, difficult spore germination, restricting the development of breeding methods, etc., to avoid contamination and high spore germination rate. , good disinfection effect

Active Publication Date: 2019-07-19
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the study of the Ussuriwawee tissue culture method, there are problems such as long cultivation period, difficult spore germination, and difficult rooting, etc. These problems restrict the development of Ussuriwawee propagation method

Method used

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  • Rapid propagation method of Lepisorus ussuriensis
  • Rapid propagation method of Lepisorus ussuriensis
  • Rapid propagation method of Lepisorus ussuriensis

Examples

Experimental program
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Effect test

Embodiment 1

[0041] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0042] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 24 hours, disinfect with 75% ethanol for 30 seconds, rinse with sterile water for 3 times, and then use a volume fraction of 0.1% HgCl solution After 8 minutes of disinfection, rinse with sterile water for 3 times before use, without breaking the sporangia during the whole process;

[0043] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the induction ...

Embodiment 2

[0053] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0054] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 20 hours, disinfect with 75% ethanol for 40 seconds, rinse with sterile water for 3 times, and then use a volume fraction of 0.1% HgCl solution After 8 minutes of disinfection, rinse with sterile water for 3 times before use, without breaking the sporangia during the whole process;

[0055] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the induction ...

Embodiment 3

[0065] (1) Obtaining explants: Take the leaves of Ussurivawe with yellow-green sporangia, gently flick off the impurities, put them in an envelope, dry them in a cool and ventilated place for 2 days, and store them in a refrigerator at 4°C for later use. The whole process keeps the sporangia intact;

[0066] (2) Disinfection of explants: soak the explants obtained in step (1) with sterile water for 30 hours, sterilize them with 75% ethanol for 20 seconds, rinse them with sterile water for 5 times, and then use a volume fraction of 0.1% HgCl solution After 8 minutes of disinfection, rinse with sterile water for 5 times before use, without breaking the sporangia during the whole process;

[0067] (3) Induce germination: Scrape off the sporangia of the Ussurivawe leaves with sporangia after disinfection in step (2), scrape off the sporangia with a clean blade, place them in sterile water, and dip a clean brush in sterile water to gently Points are cultured on the surface of the ...

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Abstract

The invention discloses a rapid propagation method of Lepisorus ussuriensis. A detached regenerated plant of Lepisorus ussuriensis is acquired via the series of steps, such as explant acquisition, explant disinfecting, induced germination, and induced rooting; rapid propagation of Lepisorus ussuriensis is achieved. The rapid propagation method of Lepisorus ussuriensis has the advantages of short cultivation cycle, high spore germination rate, high rooting rate, and low production cost, and is good for the large-scale production of Lepisorus ussuriensis.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a rapid propagation method of Usurivavir. Background technique [0002] Ussurivave is a plant of the genus Vavee in the family Pteridaceae, which has strong anti-inflammatory, analgesic, antibacterial, anti-oxidative, liver-protecting, and cough-relieving effects. Ussuriwawe mainly comes from the wild, but there are very few wild resources, which cannot meet the growing needs of human beings. Only by solving the technical difficulties of rapid reproduction can the effective protection and sustainable use of resources be realized. However, there is no report on the method of artificially cultivating Ussurivawe at present. [0003] The common propagation methods of ferns include spore propagation, branch propagation and tissue culture. The competitiveness and tolerance of spores in the natural environment are poor, and the germination rate is low, so they cannot be widely u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王丽红张春萌陈尚君崔红赵龙张义秀
Owner JIAMUSI UNIVERSITY
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