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Anti-il1-rap antibodies

一种抗体、抗体轻链的技术，应用在46.8G1,7.7A9领域

Active Publication Date: 2019-07-19
CANTARGIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The risk of recurrence remains, as surviving CSCs are able to generate new cancer cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0642] Production of antibodies against IL1-RAP

[0643] A cDNA fragment of human IL1-RAP encoding the extracellular domain (residues 21-367 of Q9NPH3 ("Q9NPH3-4")) was cloned from the full-length human IL1-RAP cDNA (Open Biosystems). A 6xHis tag was added to the C-terminus to generate IL1-RAP-His fusion protein, which was used to immunize mice to generate monoclonal mouse anti-human IL1-RAP antibody. Hybridomas were generated using standard protocols. Briefly, 4-6 week old Balb / c mice were immunized with purified recombinant IL1-RAP fusion protein twice a week for a total of 4 weeks. Titers were then assessed and splenocytes were fused with SP2 / 0 cells. Hybridomas were picked and supernatants from resulting clones were screened by enzyme-linked immunosorbent assay (ELISA) and fluorescence-activated cell sorting (FACS).

[0644] Specificity of selected hybridoma clones tested by FACS binding to parental or human IL1-RAP transfected 293 / 293IL1-RAP cells and various AML cell ...

Embodiment 2

[0646] Generation of antibodies against the membrane-bound form of IL1-RAP

[0647] To generate antibodies specific for recognition of membrane-bound IL1-RAP, different chimeric mouse / human IL1-RAP proteins were generated. Mice were immunized with each chimera and ELISA-based screening was used to screen for membrane-bound ILRAP-specific mAbs. The schematic is shown in figure 2 middle.

[0648] 2a. Identification of antibodies that specifically bind membrane-bound IL1-RAP

[0649] Antibody clones that preferentially recognize membrane-bound IL1-RAP were identified by indirect ELISA using the his-tagged extracellular domain (ECD) of IL1-RAP (Q9NPH3-4) and the soluble IL1-RAP form (Q9NPH3-2). Clones were detected using a peroxidase-conjugated goat anti-mouse IgG (subclass IgGl, 2 and 3 specific) antibody.

[0650] 2b. 2H8 preferentially recognizes membrane-bound IL1-RAP by ELISA

[0651] The extracellular domain of IL1-RAP (Q9NPH3-4) and the soluble IL1-RAP form (Q9NPH...

Embodiment 3

[0657] Binding of IL-RAP to primary AML samples

[0658] IL1-RAP clones were tested for binding to AML primary patient samples. A total of 12 AML patient samples were examined with clones 2H8, 50.3G7 and 56.12G6, all antibodies were directed to the surface expressed protein of IL1-RAP. 2H8 recognized 6 / 12 (50%) of the CD34+ population of patient AML patient samples. These AML patient samples were from different 2008 WHO and FAB classifications. Figures 6A-6D show exemplary FAC data for individual positive patients.

[0659] Expression analysis by flow cytometry. BMMC or PBMC were isolated from bone marrow aspirates or whole blood by Ficoll separation (GE Healthcare). Cells were resuspended in staining solution (HBSS, 1% BSA, 1 mM EDTA) to 2x10 6 cells / mL and blocked with mouse, rat or human IgG before staining. Add 5 μL of CD34-APC to each tube with 50 μL of staining solution. Add various antibodies mIgG, 2H8, 56.12G6, 50.3G7 antibodies to each tube to a final concentra...

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Abstract

Provided herein are antibodies specific for IL1-RAP.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Provisional Application No. 62 / 408,807, filed October 16, 2016, U.S. Provisional Application No. 62 / 425,970, filed November 23, 2016, and International Application, filed October 10, 2017 The benefit of the filing date of PCT / US2017 / 055994, all of which are hereby incorporated by reference in their entirety. Background technique [0003] Interleukin-1 (IL1) is a key regulator of acute and chronic inflammatory responses mediated by the immune system. Interleukin-1 receptor accessory protein (IL1-RAP, also known as IL-1R3) is a co-receptor for IL1 that functions in the IL1 receptor signaling complex and, in some cases, is essential for linking cell membrane events with downstream signaling. A via connection is necessary. IL-1 plays an important role in mammalian immunity by inducing the synthesis of acute phase and proinflammatory proteins during infection, tissue injury and stress. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/00C07K16/28
CPCC07K16/2866A61K2039/505C07K2317/24C07K2317/30C07K2317/76A61K47/6849A61P31/00A61K47/6801G01N33/6857
Inventor 蒋英萍杰格塔·R·祖奴图拉伦纳德·G·普雷斯塔
Owner CANTARGIA
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