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Determination method based on residual amount of MTMC in animal derived food

A determination method and technology for residues, which can be applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as health threats, increased consumption of carbamaze, vegetables, water sources and environmental pollution, and achieve good accuracy, high sensitivity, Avoid the effects of reduced chromatographic separation power

Inactive Publication Date: 2019-07-30
厦门泓益检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the prohibition of some highly toxic pesticides such as methamidophos, the amount of Memocarb has increased greatly, which has polluted food, vegetables, water sources and the environment, and has a potential threat to human health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The acetonitrile solution can be replaced by 20-30ml n-hexane solution.

[0036] A method based on the determination of medicarb residues in kinetic food, including 200g pork, 3-5g sodium chloride, 20-30ml n-hexane solution or 20-30ml acetone solution, 300-450mgPSA, 300-450mgC18, 30 -50mgGCB, gas chromatograph (with nitrogen and phosphorus detector), centrifuge, nitrogen blowing instrument, vortex shaker and homogenizer, are characterized in that comprising the following steps:

[0037] 1) Take edible pork as the measuring object, and prepare the samples;

[0038] ①Peel 200g pork, remove the fat, and take the lean part;

[0039] ②Cut the pork into small pieces and mix well;

[0040] 2) Purify the sample

[0041] ① Add 20-30ml of acetonitrile solution to 5g of the homogeneously mixed sample, after homogeneous extraction for 2 minutes, add 3-5g of sodium chloride, vortex for 5 minutes to extract, and centrifuge at 5000 rpm for 10 minutes at -4°C;

[0042] ② Take all t...

Embodiment 2

[0047] The acetonitrile solution can be replaced by 20-30ml acetone solution.

[0048] A kind of assay method based on medicarb residue in kinetic food, comprising 200g pork, 3-5g sodium chloride, 20-30ml acetone solution, 300-450mgPSA, 300-450mgC18, 30-50mgGCB, gas chromatograph ( band nitrogen phosphorus detector), centrifuge, nitrogen blowing instrument, vortex shaker and homogenizer, it is characterized in that comprising the following steps:

[0049] 1) Take edible pork as the measuring object, and prepare the samples;

[0050] ①Peel 200g pork, remove the fat, and take the lean part;

[0051]②Cut the pork into small pieces and mix well;

[0052] 2) Purify the sample

[0053] ① Add 20-30ml of acetonitrile solution to 5g of the homogeneously mixed sample, after homogeneous extraction for 2 minutes, add 3-5g of sodium chloride, vortex for 5 minutes to extract, and centrifuge at 5000 rpm for 10 minutes at -4°C;

[0054] ② Take all the supernatant, add 300-450mgPSA (ethyle...

Embodiment 3

[0059] A method based on the determination of medicarb residues in kinetic food, including 200g pork, 3g sodium chloride, 20ml acetonitrile solution, 3000mgPSA, 300mgC18, 30mgGCB, gas chromatography (with nitrogen and phosphorus detector), centrifuge, Nitrogen blowing instrument, vortex oscillator and homogenizer are characterized in that comprising the following steps:

[0060] 1) Take edible pork as the measuring object, and prepare the samples;

[0061] ①Peel 200g pork, remove the fat, and take the lean part;

[0062] ②Cut the pork into small pieces and mix well;

[0063] 2) Purify the sample

[0064] ① Add 20ml of acetonitrile solution to 5g of the homogeneously mixed sample, after homogeneous extraction for 2 minutes, add 3g of sodium chloride, vortex for 5 minutes to extract, and centrifuge at 5000 rpm for 10 minutes at -4°C;

[0065] ② Take all the supernatant, add 300mgPSA (ethylenediamine-N-propylalkane), 300mgC18, 30mgGCB (graphitized carbon black), vortex for 60s...

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Abstract

The invention provides a determination method based on the residual amount of MTMC in animal derived food, and relates to the technical field of determination of the residual amount of MTMC. Accordingto the method, residual pesticides through extraction, purification and concentration is injected into a gas chromatograph, different residual pesticides are separated in a solid phase, a gas chromatogram is drawn through detection and scanning of a nitrogen phosphorus detector, qualitative determination is performed through a retention time, quantification is performed through comparison of a peak or a peak area and a standard curve, multiple components can be simultaneously determined at one time, and the method has characteristics of simplicity, rapidity, high sensitivity and good accuracy. According to the method, samples with complex matrixes or many impurities are purified through a solid-phase extraction purification mode, and interference components in a co-extract after purification can be effectively removed in a chromatographic condition so that a sample inlet or a column head is prevented from being polluted by the impurities or the phenomenon that the impurities occupy active sites of a gas chromatograph analysis system is avoided, and phenomena of reduced chromatographic separation capability, severe interference and reduced instrument sensitivity are avoided.

Description

technical field [0001] The invention relates to the technical field of measuring the residual amount of amecarb, in particular to a method for determining the residual amount of amecarb in kinetic food. Background technique [0002] Memecarb is a carbamate-type pesticide, which mainly acts as contact and fumigant, inhibits the activity of cholinesterase in insects, and leads to the accumulation of acetylcholine poisoning and death. Occupational acute amecarb poisoning, the poison intrusion route is mainly through respiratory inhalation and skin absorption poisoning. Methiocarb remains on the crops mainly in the form of the body, and the residual concentration is related to the frequency of application, harvest time, time of the last application and the variety of crops. [0003] The study of Methiocarb on mice found that a limited number of exposures to Meprocarb in the early postnatal period can delay the appearance of two physiological developmental signs, the eruption of...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/62
CPCG01N30/06G01N30/62
Inventor 廖军海陈妙瑞陈东海
Owner 厦门泓益检测有限公司
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