Multi-index detection microfluidic card box and application method

Abstract

The invention discloses a multi-index detection microfluidic card box and an application method. The multi-index detection microfluidic card box comprises a piston pump, liquid storage pipes, a liquidstorage pipe cover, a card box base, a cover plate, a sealing gasket, a gate valve and a base plate, wherein the piston pump performs pushing and pulling to drive fluid in the card box; the gate valve rotates to open and close runner connection; the liquid storage pipes store reagent samples and are sealed by the liquid storage pipe cover; the card box base is in supported connection with all components; the cover plate seals a flow path pipeline and a cavity on the card box base; the sealing gasket is arranged and sealed between the card box base and the gate valve; the base plate assemblesthe gate valve on the card box base through a fixing member. The card box integrates the pump and the valve driven by fluid as well as liquid required by reaction, has no flow path connection with theoutside and can realize multi-index automatic detection and analysis of nucleic acid, protein and biochemistry for biological samples. The multi-index detection microfluidic card box has the advantages of simple operation flow, abundant detection results and high clinical application value.

Description

Technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a multi-index detection microfluidic cartridge and an application method. Background technique [0002] Fully integrated automated analysis is the development trend in the field of in vitro diagnostics. For molecular diagnosis in in vitro diagnostics, integrated automated nucleic acid detection technology is the development trend of molecular diagnostic technology. Its important significance is not only to solve the problem of manpower and laboratory space occupation, but also to avoid the harm of samples to operators and step-by-step operations Bringing problems such as potential product contamination can effectively improve the reliability of clinical diagnosis and reduce costs. For immunological and biochemical diagnosis in in vitro diagnostics, fully automatic multi-indicator joint detection is the development direction. It can not only save manpower and material resou...

AI Technical Summary

Problems solved by technology

[0003] The three basic types of reactions required for in vitro diagnostics require a wide variety of reagents, cumbersome steps, and complex liquid manipulation and detection devices.

Therefore, the instruments and equipment used for in vitro diagnostic testing often require more liquid control components, making the equipment bulky, and it is difficult to meet the needs of areas with backward medical infrastructure and on-site real-time testing

Moreover, the sample processing process often cannot be flexibly changed according to actual needs, and the types and volumes of samples to be processed are relatively small.

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