mos 2 QDs fluorescent probe, its synthesis method and application

A fluorescent probe and synthesis method technology, applied in the field of biological fluorescent probes and transition metal sulfide quantum dot biological fluorescent probes, can solve the problems of easy oxidation, low quantum yield of fluorescent probes, and cumbersome production process of fluorescent probes etc. to achieve strong anti-corrosion properties, improve anti-oxidation and photo-bleaching properties, and excellent biocompatibility

Active Publication Date: 2022-03-11
CHAOHU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quantum yield of these fluorescent probes is often not high, and the production process of fluorescent probes is cumbersome
The surface of graphene quantum dots contains a large number of C=C double bonds, which are easy to oxidize, and its oxidation resistance and photobleaching performance need to be improved

Method used

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  • mos <sub>2</sub> QDs fluorescent probe, its synthesis method and application
  • mos <sub>2</sub> QDs fluorescent probe, its synthesis method and application
  • mos <sub>2</sub> QDs fluorescent probe, its synthesis method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] (1)MoS 2 Preparation of quantum dot powder

[0061] 1) Dissolve 0.225 g and 0.13 g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36 mL of deionized water, and adjust the pH value of the mixed solution to 7 with ammonia water or hydrochloric acid.

[0062] 2) After the above mixed solution was sonicated for 25 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 200° C., and reacted for 16 hours.

[0063] 3) After the reaction is completed, the reactor is left to stand for 5 minutes. Take the supernatant suspension, and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes at a high speed. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0064] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solution...

Embodiment 2

[0081] (1)MoS 2 Preparation of quantum dot powder

[0082] 1) Dissolve 0.338g and 0.13g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36mL deionized water respectively. Adjust the pH value of the mixed solution to 8 with ammonia water or hydrochloric acid.

[0083] 2) After the above mixed solution was sonicated for 20 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 210° C., and reacted for 14 hours.

[0084] 3) After the reaction is finished, the reactor is left to stand for 10 minutes. Take the supernatant suspension and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes at a high speed. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0085] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solut...

Embodiment 3

[0093] (1)MoS 2 Preparation of quantum dot powder

[0094] 1) Dissolve 0.45 g and 0.13 g of cystamine dihydrochloride and ammonium tetrathiomolybdate in 36 mL of deionized water, and adjust the pH value of the mixed solution to 9 with ammonia water or hydrochloric acid.

[0095] 2) After the above mixed solution was sonicated for 15 minutes, it was transferred into a 50 mL stainless steel reaction kettle with a polytetrafluoroethylene liner, heated at 220° C., and reacted for 12 hours.

[0096] 3) After the reaction is completed, the reactor is left to stand for 15 minutes. Take the supernatant suspension and use a high-speed centrifuge to centrifuge at a speed of 10,000 n / min for 20 minutes at a high speed. After centrifugation, the supernatant is filtered with a filter membrane with a pore size of 0.22 μm to obtain a solution.

[0097] 4) Dialyze the solution obtained in the above steps with a dialysis bag with a molecular weight cut-off of 1000, put the dialyzed solution ...

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Abstract

The invention discloses a MoS for detecting hemoglobin 2 The synthetic method of QDs fluorescent probe comprises the following steps: (1) dissolving cystamine dihydrochloride and ammonium tetrathiomolybdate in deionized water to obtain a mixed solution with a pH value of 7-11; (2) dissolving the above-mentioned After the mixed solution is ultrasonically heated, the reaction is carried out; (3) after the reaction is completed, let it stand still, and the supernatant is taken for centrifugation; (4) after centrifugation, the supernatant is filtered to obtain a filtered solution; (5) the filtered solution is dialyzed in a vacuum Dry to get MoS 2 QDs powder; (6) MoS using step (5) 2 Preparation of MoS from QDs powder 2 Aqueous solution of QDs as MoS for detection of heme 2 QDs fluorescent probes. The present invention also discloses MoS 2 QDs fluorescent probes and applications. The fluorescent molybdenum disulfide quantum dot provided by the invention has the advantages of good biocompatibility, good stability, high fluorescence quantum yield, simple synthesis conditions, and low price. When it is used to detect hemoglobin, the detection process is simple and the sensitivity is high. .

Description

technical field [0001] The invention relates to the technical field of biological fluorescent probes, in particular to the technical field of transition metal sulfide quantum dot biological fluorescent probes. Background technique [0002] High or low hemoglobin in human blood (hereinafter referred to as hemoglobin) can cause various diseases and even endanger life. It is of great significance to detect heme in human body and functional food. Organic dyes and fluorescent proteins are used as fluorescent probes to test heme, but their fluorescence intensity is low and the emission spectrum is wide. Traditional II-VI, III-V quantum dot fluorescent probes are used, which contain heavy metals such as Cd and Pb, which are highly toxic to organisms. Materials such as carbon quantum dots and graphene quantum dots can be modified by coupling with antibodies or polypeptides to obtain better biocompatibility. However, the quantum yield of these fluorescent probes is often not high,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/68B82Y20/00B82Y40/00G01N21/64
CPCC09K11/681B82Y20/00B82Y40/00G01N21/6428G01N21/6486G01N2021/6432
Inventor 吴凤义王新运李川高玉荣李明玲高立王志海邵娟娟武梦婷赵孝郑陈晨吴梦晴吴凤彬
Owner CHAOHU UNIV
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