Human umbilical cord mesenchymal stem cell-derived stem cell factor microvesicle preparation and preparation method

A stem cell factor and stem cell technology, which is applied in the field of preparation and preparation of human umbilical cord mesenchymal stem cell-derived stem cell factor microvesicles, can solve the problem of limited total amount of microvesicles, influence of product uniformity, and influence on the preparation of microvesicles on cell state. and other problems, to avoid medical ethics problems, to achieve good product uniformity, and to facilitate large-scale production.
CN110179826BActive Publication Date: 2021-08-31武汉五州润达生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
武汉五州润达生物医药科技有限公司
Publication Date
2021-08-31

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Abstract

The invention relates to a human umbilical cord mesenchymal stem cell-derived stem cell factor microvesicle preparation and a preparation method. The method uses the umbilical cord tissue discarded by clinical pregnant women after childbirth as a material to obtain human umbilical cord mesenchymal stem cells and human umbilical cord mesenchymal stem cells with medical value Stem cell factor microvesicles derived from stem cells have good product uniformity and high production efficiency, which is conducive to large-scale production and avoids medical ethics issues. This method strictly operates in the process of culturing and expanding human umbilical cord mesenchymal stem cells. The whole process is carried out in a million-level cell laboratory without using antibiotics, which avoids possible changes in stem cell properties and damage to human umbilical cord mesenchymal stem cells. The influence of the microvesicle quality of the source stem cell factor can trigger tissue allergic reactions; use optimized serum-free human umbilical cord mesenchymal stem cell culture medium to avoid animal-derived blood components mixed into the suspension and cause human tissue allergy; the frozen microvesicle suspension prepared Dry powder is easy to store and provides convenience for subsequent product production.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a human umbilical cord mesenchymal stem cell-derived stem cell factor microvesicle preparation and a preparation method. Background technique

[0002] Human umbilical cord mesenchymal stem cells have biological functions such as tissue repair and immune regulation, but because external use of human umbilical cord mesenchymal stem cells may cause blockage of skin pores and sebaceous glands, local skin inflammation and even skin allergies, as well as long-term abnormal differentiation and tumor formation, etc. Risk, resulting in its limited clinical application in plastic surgery, especially in skin repair. Human umbilical cord mesenchymal stem cell-derived stem cell factor microvesicles are produced by human umbilical cord mesenchymal stem cells under a special induction state, which can effectively simulate the functional membranous spherical structure of human umbilical cord mesenchym...

Claims

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