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A kind of renewable acetamiprid electrochemical sensor and its preparation method and application

A kind of acetamiprid, electrochemical technology, applied in the field of electrochemical biosensor

Inactive Publication Date: 2021-07-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The technical problem to be solved in the present invention is to firmly immobilize the DNA chain on the surface of the electrode through the special design of the DNA sequence and structure, and at the same time make the sensor have dual functions of identification and signal; it is particularly important to enable the sensor to recognize acetamiprid. Quickly restore the original state, with the characteristics of regeneration and repeated use

Method used

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  • A kind of renewable acetamiprid electrochemical sensor and its preparation method and application

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Effect test

Embodiment 1

[0030] Example 1 "T" DNA structure used in acetamiprid electrochemical biosensor

[0031] The "T"-shaped DNA strand is an asymmetric hairpin structure with dual functions of recognition and signaling. Methylene blue MB label; the 5' end of the recognition chain and the 3' end of the signal chain are connected by thymine T, and thymine T is grafted with a sulfhydryl group; its structure is: 5'MB-TGCAGACAAATTACA-3'-T(C 6 -SH)-5'-TGTAATTTGTCTGCAGCGGTTCTTGATCGCTGACACCATATTATGAAGA-3';

Embodiment 2

[0032] Example 2 Preparation of gold-modified glassy carbon electrode Au / GCE used in acetamiprid electrochemical biosensor

[0033] The glassy carbon electrode was coated with 0.05 μm Al 2 o 3 The suspension was ground and polished on a polishing cloth, and then ultrasonically cleaned twice with deionized water. Place the treated glassy carbon electrode as a working electrode in a solution containing 2.5mM HAuCl 4 , 0.5M H 2 SO 4 1. In the electrolyte solution of 150mM ethylenediamine, the Ag / AgCl electrode is used as the reference electrode, and the platinum electrode is used as the auxiliary electrode, and the constant potential deposition is carried out at 0.0V for 600s;

Embodiment 3

[0034] Example 3 Preparation of a Renewable Acetamiprid Electrochemical Biosensor

[0035] On the gold-modified glassy carbon electrode Au / GCE, drop 5 mL of 0.01 μM "T" DNA chain, and incubate in a vacuum oven at 40 °C for 1 h; block non-specific binding with 5 mL of 10 mM 6-mercaptohexanol MCH The site was washed three times with 0.1M PBS solution with pH 7.4 to prepare a DNA / Au / GCE electrode, which was stored in the refrigerator at 4°C for later use;

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Abstract

The present invention relates to the technical field of electrochemical biosensors, in particular to a reproducible method for preparing an electrochemical biosensor for the detection of pesticide residue acetamiprid, including the preparation steps of the acetamiprid electrochemical biosensor, and using the sensor to measure acetamiprid. The operation method and regeneration method of acetamiprid, etc., this renewable acetamiprid electrochemical sensor is easy to make, the sensor is easy to regenerate, can be used repeatedly, has good reproducibility, is non-toxic, does not pollute the environment, and this determination method is selected Good performance and high sensitivity.

Description

technical field [0001] The present invention relates to the technical field of electrochemical biosensors, in particular to a reproducible method for preparing an electrochemical biosensor for the detection of pesticide residue acetamiprid, including the preparation steps of the acetamiprid electrochemical biosensor, using the sensor to measure pyridine The operation method of chrysanthemum, and the regeneration method, etc. Background technique [0002] While organic pesticides provide guarantee for agricultural production, their residues also cause harm to the environment and human health. Pesticide residues will not only pollute soil, water bodies, etc., causing environmental degradation, but more seriously, will directly endanger people's health and even life safety. Therefore, the development of rapid detection technology with high sensitivity, high stability and specific response to pesticides and their metabolites is undoubtedly of great significance to national heal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/3275
Inventor 周长利刘建辉贾玉秀陈培培夏方诠田栋
Owner UNIV OF JINAN
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