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Method of establishing autotetraploid carp line and method of breeding autotriploid carp

A technology of autotetraploid and establishment method, which is applied in the field of distant hybridization of fish, can solve the problems of low hatching rate and seedling survival rate, achieve high hatching rate and seedling survival rate, fast growth, genetic clear background effect

Active Publication Date: 2019-09-06
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the hybrid combination with carp as the female parent and bream as the male parent can survive, its hatching rate and seedling survival rate are low, and the hybrid offspring has not been able to form a genetically stable tetraploid line

Method used

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  • Method of establishing autotetraploid carp line and method of breeding autotriploid carp
  • Method of establishing autotetraploid carp line and method of breeding autotriploid carp
  • Method of establishing autotetraploid carp line and method of breeding autotriploid carp

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Embodiment 1

[0047] A method for establishing an autotetraploid carp strain of the present invention, comprising the steps of:

[0048] 1. Selection and cultivation of parents: 3 to 4 months before the breeding period, the omnivorous carp was selected as the original female parent, and the herbivorous bream was selected as the original male parent. 1 Selected from the generation have obvious characteristics of sexual maturity (female belly swells, male fish can squeeze out milky white semen by lightly pressing the abdomen), good body shape, bright body color, strong physique, no disease and no injury (no large area of ​​scales falling off, all parts of the body No obvious scars, etc.) female allotetraploid bream (4n=148) (outline photo see figure 1 ) and male homodiploid crucian carp (2n=100) (see figure 2 ), and raise them in special ponds to keep the water quality good; before the artificial induction of labor, the parent fishes of the father and mother were put into the broodstock pon...

Embodiment 2

[0067] A method for breeding autotriploid carp using the autotetraploid carp obtained in Example 1, specifically comprising the following steps:

[0068] 1. Selection and cultivation of parents: 3 to 4 months before the breeding season, select animals with obvious characteristics of sexual maturity (swollen abdomen or lightly squeezed abdomen can squeeze out milky white semen), good body shape, bright color, strong physique, and no disease. Female diploid carp (2n=100) and male autotetraploid carp (4n=200) without injury (no extensive scale loss, no obvious scars on various parts of the body, etc.) image 3 ), and raise them in special ponds to keep the water quality good; before the artificial induction of labor, the parent fishes of the father and mother were put into the broodstock ponds for 3 to 4 months, and the water quality was kept good, especially before the breeding period. Feed with fine bait for about a month, and stimulate with running water every 2 to 3 days to p...

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Abstract

The invention discloses a method of establishing an autotetraploid carp line. The method comprises hybridizing with a carp as an original female parent and a Wuchang bream as an original male parent,selecting a female allotetraploid carp-bream and a male autodiploid carp from a hybrid generation F1, carrying out artificial spawning, carrying out artificial dry insemination, hatching with runningwater, feeding with a special pond, testing, screening to obtain an autotetraploid carp generation F1 having chromosome number of 200, subjecting male and female autotetraploid carp generations F1 tomulti-generation selfing subculture to obtain the autotetraploid carp line having stable genetic character. The autotetraploid carp line acquired herein has the advantages of beautiful body form, highgrowth speed, good stress tolerance, good reproducibility and the like; both hatching rate and seed survival rate are high; both sexes are fertile, and genetic stability is good. A method of subjecting a diploid carp as a female parent and an autotetraploid carp as a male parent to interploidy hybridization to obtain autotriploid carps. The triploid carps acquired herein have the advantages of potential infertility, high growth speed, good meat quality and the like, and have important value in production and application.

Description

technical field [0001] The invention belongs to the field of distant hybridization of fish, and in particular relates to a method for establishing an autotetraploid carp strain and a method for breeding an autotetraploid carp. Background technique [0002] As an important method of fish genetic breeding and trait improvement, distant hybridization can integrate a whole set of exogenous genes, thereby changing the genetic composition of the genetic structure of the hybrid offspring and the regulation of gene expression, and even changing the ploidy level of the hybrid offspring and eventually Establishment of genetically stable tetraploid populations. In fish genetics and breeding, methods such as cold / heat shock can usually be used to inhibit the first mitosis of fertilized eggs to prepare autotetraploid fish, but the resulting autotetraploid fish often have abnormal gonad development or Parthenofertile, unable to form a stable tetraploid fish line due to unstable fertility...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 刘少军王石罗凯坤胡方舟周佩吴昌龚丁斌张纯陶敏覃钦博姚占洲赵如榕
Owner HUNAN NORMAL UNIVERSITY
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