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Medicinal wild rice oomyb2 protein and its coding gene, expression vector and application

A technology of medicinal wild rice and protein, applied in the field of genetic engineering, can solve the problems of ignoring drought resistance, cold resistance, loss of excellent resistance genes, etc.

Active Publication Date: 2021-06-29
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of artificial selection, breeders often pay more attention to decisive traits such as yield and quality, and ignore secondary traits such as drought resistance and cold resistance, which has led to the loss of many excellent resistance genes in cultivated rice.

Method used

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  • Medicinal wild rice oomyb2 protein and its coding gene, expression vector and application
  • Medicinal wild rice oomyb2 protein and its coding gene, expression vector and application
  • Medicinal wild rice oomyb2 protein and its coding gene, expression vector and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Bioinformatics analysis of the medicinal wild rice MYB family

[0070] In order to analyze the characteristics of the MYB gene family of O. 154 transcripts can be annotated as R2R3-MYB and MYB-related transcription factor families. Sequence analysis revealed that 54 transcripts had complete coding regions and belonged to the R2R3-MYB transcription factor gene family. The protein structure of R2R3-MYB transcription factor family genes is characterized by two or more SANT conserved domains, which are protein-DNA binding domains. Use the PFAM website to obtain the amino acid sequence of the SANT conserved domain of its protein, and further use the MEME software to analyze the amino acid sequence of the SANT conserved domain obtained, the results are as follows figure 1 Shown, wherein, A is R2, B is R3.

[0071] In order to analyze the difference between the amino acid sequence of the SANT conserved domain of the medicinal wild rice R2R3-MYB family protein a...

Embodiment 2

[0072] Embodiment 2: Gene screening and analysis

[0073] In this study, the differential expression analysis of genes was performed based on the expression abundance value RPKM value of each sample gene in the transcript profiling sequencing (RNA-seq) data of the four-leaf stage medicinal wild rice treated with 20% PEG simulated drought and the untreated group. In order to find genes related to drought stress, the differential gene screening conditions were set as: q-value1, and finally 743 genes related to drought stress in medicinal wild rice were obtained. After analyzing the annotation information, it was found that unigenes belonged to the plant MYB transcription factor family numbered comp101333_c0 and named OoMYB2. Its nucleotide sequence was shown in SEQ ID No.2, and the complete coding region of this gene was successfully detected by transcriptional profiling sequencing. Data analysis showed that the RPKM values ​​in the untreated and treated groups were as follows...

Embodiment 3

[0074] Example 3: Analysis of medicinal wild rice OoMYB2 protein

[0075] The present invention provides a medicinal wild rice OoMYB2 protein, which is a protein composed of the amino acid sequence shown in SEQ ID No.1; or the amino acid sequence shown in SEQ ID No.1 is substituted, deleted or increased by 1 to 3 amino acid derivatives.

[0076] The amino acid sequence shown in SEQ ID NO.1 is as follows:

[0077]

[0078] The amino acid sequence is 304 in length, has a SANT structure (position: amino acid residues 123-173), and a ZnF_C2HC domain (position: amino acid residues 12-28); and its homologous protein amino acid difference sequence LOC_Os01g09640 The similarity is 93.2%; sequence comparison such as figure 2 shown.

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Abstract

The invention discloses a medicinal wild rice OoMYB2 protein, which is a protein composed of the amino acid sequence shown in SEQ ID No.1 or a derivative protein thereof. The present application also provides the gene encoding the OoMYB2 protein, especially the gene shown in SEQ ID No.2. The invention also provides an expression carrier of the gene, and the application of the medicinal wild rice OoMYB2 protein in the breeding of cultivated rice varieties and the application of improving the drought resistance ability of cultivated rice. The SEQ ID No.2 provided by the invention is a unique favorable gene of medicinal wild rice, and increasing the expression of OoMYB2 protein can improve the drought resistance ability of cultivated rice.

Description

technical field [0001] The invention relates to a genetic engineering, in particular to the medicinal wild rice OoMYB2 protein, its coding gene, expression vector and application. Background technique [0002] Drought is a frequent natural disaster and a common adversity stress in rice cultivation, which seriously affects the germination, growth, and fruiting of rice. In recent years, with global warming, drought stress has appeared more frequently in rice cultivation. [0003] Cultivated rice is obtained from wild rice through long-term artificial selection. In the process of artificial selection, breeders often pay more attention to decisive traits such as yield and quality, and ignore secondary traits such as drought resistance and cold resistance, which leads to the loss of many excellent resistance genes in cultivated rice. [0004] Medicinal wild rice (Oryza officinalis Wall.) has been growing in harsh natural environments for a long time, and it carries excellent re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H4/00A01H5/00A01H6/46
CPCA01H4/00C07K14/415C12N15/8273
Inventor 李亚娟陈志雄刘向东王兰黄小捷谭宇
Owner SOUTH CHINA AGRI UNIV