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The ssr core primer set developed based on the whole genome sequence of pomegranate and its application

A whole genome and core primer technology, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial measurement/inspection, etc., to achieve good application prospects, simple methods, and high development efficiency

Active Publication Date: 2021-07-30
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] Based on the long history of pomegranate cultivation, rich resources, and frequent exchange of varieties, it is of great practical significance to carry out pomegranate variety identification, fingerprint construction, genetic diversity evaluation and phylogenetic relationship research. However, the effective development of this work depends on polymorphism. Molecular marker development with high reliability, good reproducibility and stable labeling

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  • The ssr core primer set developed based on the whole genome sequence of pomegranate and its application
  • The ssr core primer set developed based on the whole genome sequence of pomegranate and its application
  • The ssr core primer set developed based on the whole genome sequence of pomegranate and its application

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Embodiment Construction

[0023] The present invention will be further explained below in conjunction with the embodiments and the accompanying drawings. The following examples are only used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0024] 1. Pomegranate Genomic DNA Extraction

[0025] (1) Select 23 varieties of pomegranate resources from different production areas

[0026] The names and sources of the 23 pomegranate varieties are shown in Table 1.

[0027] Table 1 Information on 23 pomegranate varieties

[0028]

[0029]

[0030] (2) Genomic DNA extraction by CTAB method

[0031] Weigh 0.2-0.3g of fresh leaves, add liquid nitrogen to quickly grind into powder, transfer the powder into a 2.0mL centrifuge tube; add 1.0mL of 3×CTAB extract preheated at 65°C in a 65°C water bath for 1 hour; centrifuge at 12,000rpm at room temperature 10min; draw the supernatant into a 2.0mL centrifuge tube; add an equal volume of phenol / chloroform / is...

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Abstract

The present invention discloses the SSR core primer set developed based on the whole genome sequence of pomegranate and its application. The nucleotide sequence is shown in sequence as SEQ ID NO.1-22. The SSR core primer set of the present invention has the advantages of high polymorphism, good repeatability, stable labeling, clear and easy-to-interpret band patterns, etc., and can be used in the fields of pomegranate variety identification, DNA fingerprinting, genetic diversity evaluation and phylogenetic research, etc. It provides a new tool for molecular marker-assisted selection in pomegranate, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of molecular biology technology, specifically, the use of the whole pomegranate genome sequence to develop an SSR core primer set and its application in pomegranate variety identification, fingerprint map construction, genetic diversity evaluation and phylogenetic research. Background technique [0002] Microsatellite (Simple Sequence Repeats, SSR) markers refer to simple repeating sequences widely present in the genome with 1 to 6 nucleotides as the unit. SSR has the characteristics of wide distribution, co-dominant inheritance, high polymorphism, convenient and stable detection, and is widely used in many research fields such as variety identification, genetic diversity evaluation, phylogenetic relationship research, genetic map construction and QTLs positioning. In the early stage, the development of SSR markers was mainly based on genomic DNA libraries or libraries rich in specific microsatellites. This method has...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2600/13
Inventor 秦改花徐义流刘春燕齐永杰高正辉伊兴凯潘海发张晓玲黎积誉
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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