Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SSR (simple sequence repeat) core primer group developed based on genome-wide sequence of permanganate and application thereof

A whole genome, core primer technology, applied in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc., to achieve the effects of high polymorphism, low cost and high development efficiency

Active Publication Date: 2019-09-06
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the long history of pomegranate cultivation, rich resources, and frequent exchange of varieties, it is of great practical significance to carry out pomegranate variety identification, fingerprint construction, genetic diversity evaluation and phylogenetic relationship research. However, the effective development of this work depends on polymorphism. Molecular marker development with high reliability, good reproducibility and stable labeling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SSR (simple sequence repeat) core primer group developed based on genome-wide sequence of permanganate and application thereof
  • SSR (simple sequence repeat) core primer group developed based on genome-wide sequence of permanganate and application thereof
  • SSR (simple sequence repeat) core primer group developed based on genome-wide sequence of permanganate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The present invention will be further explained below in conjunction with the embodiments and the accompanying drawings. The following examples are only used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0024] 1. Pomegranate Genomic DNA Extraction

[0025] (1) Select 23 varieties of pomegranate resources from different production areas

[0026] The names and sources of the 23 pomegranate varieties are shown in Table 1.

[0027] Table 1 Information on 23 pomegranate varieties

[0028]

[0029]

[0030] (2) Genomic DNA extraction by CTAB method

[0031] Weigh 0.2-0.3g of fresh leaves, add liquid nitrogen to quickly grind into powder, transfer the powder into a 2.0mL centrifuge tube; add 1.0mL of 3×CTAB extract preheated at 65°C in a 65°C water bath for 1 hour; centrifuge at 12,000rpm at room temperature 10min; draw the supernatant into a 2.0mL centrifuge tube; add an equal volume of phenol / chloroform / is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an SSR (simple sequence repeat) core primer group developed based on genome-wide sequence of permanganate and application thereof. The SSR core primer group comprises 11 pairsof pairs, to be specific, PG080, PG130, PG139, PG152, PG153, PG140, PG098, PG070, PG077, PG090, and PG093; nucleotide sequences of the SSR core primer group are shown sequentially as SEQ ID NO. 1 to 22. The SSR core primer group has the advantages of high polymorphism, good repeatability, good marking stability, good clarity of banding pattern, and good convenience of interpretation and the like;the SSR core primer group is applicable to the fields of identification of permanganate species, DNA fingerprinting, evaluation of genetic diversity, systematic development research and the like; a novel tool is provided for molecular marker-assisted selection of permanganate; the SSR core primer group has good application prospect.

Description

technical field [0001] The invention relates to the field of molecular biology technology, specifically, the use of the whole pomegranate genome sequence to develop an SSR core primer set and its application in pomegranate variety identification, fingerprint map construction, genetic diversity evaluation and phylogenetic research. Background technique [0002] Microsatellite (Simple Sequence Repeats, SSR) markers refer to simple repeating sequences widely present in the genome with 1 to 6 nucleotides as the unit. SSR has the characteristics of wide distribution, co-dominant inheritance, high polymorphism, convenient and stable detection, and is widely used in many research fields such as variety identification, genetic diversity evaluation, phylogenetic relationship research, genetic map construction and QTLs positioning. In the early stage, the development of SSR markers was mainly based on genomic DNA libraries or libraries rich in specific microsatellites. This method has...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2600/13
Inventor 秦改花徐义流刘春燕齐永杰高正辉伊兴凯潘海发张晓玲黎积誉
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com