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Rhizosphere Simulation Cultivation Device and Its Application

A rhizosphere soil and nutrient technology, applied in the field of soil science, can solve the problems of restricting root growth, lack of oxygen, restricting cylindrical pots, etc., and achieve the effect of avoiding unidirectional growth, simple production, and avoiding uneven nutrients

Active Publication Date: 2018-12-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in cylindrical pots, the edge is better ventilated and the root system is dense, while the lack of oxygen in the center of the soil restricts the growth of the root system, thereby limiting the use of cylindrical pots in the study of rhizosphere effects

Method used

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  • Rhizosphere Simulation Cultivation Device and Its Application
  • Rhizosphere Simulation Cultivation Device and Its Application
  • Rhizosphere Simulation Cultivation Device and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] See figure 1 The rhizosphere simulation culture device mainly includes a basin 1, a hollow tube 2, a polyethylene mesh 3. The hollow tube 2 is inserted into the basin 1 obliquely, and the hollow tube 2 and the basin 1 present an angle of 75-85°. The net 3 is tightly wrapped around the hollow tube 2.

[0019] Hollow tube 2 is provided with dense small holes, the size of the pores is 0.5-5.0mm, the size and quantity of the pores are determined by the soil texture, the more viscous the soil texture, the larger the pore size, the more the number, the hollow tube is wrapped with a layer of pore size It is a 24 micron polyethylene net, the hollow tube mainly plays the role of support and ventilation. The 24 micron polyethylene net allows air and soil solution to pass through freely, but the root system cannot penetrate.

[0020] The thickness of the hollow tube 2 depends on the size of the pot, the soil texture and the growth of the root system of the plant. If the pot is larger,...

Embodiment 2

[0021] Example 2 Application of rhizosphere simulation culture device in the research of rhizosphere soil nutrient turnover changes

[0022] Collect Xianju high mountain soil, naturally air-dry to 15% water content, pass the soil through a 3mm sieve, add 260g of soil to pot 1, the height of the pot is 12cm, the diameter of the upper part is 6cm, the diameter of the bottom is 5cm, and the pot is installed 6 polyethylene pipes with a length of 3cm and a diameter of 1cm, containing dense 0.5mm holes, the polyethylene pipes are wrapped with a layer of 24 micron polyethylene mesh. Two corn plants were planted in each pot. After 25 days of planting, the pot was cut and the polyethylene hollow tube was taken out from the soil. Root samples were collected from the soil. The root morphology was measured using a root scanner (STD1600, Epson, Nagano, Japan). ) Perform measurement and analysis.

[0023] A traditional square rhizosphere simulator with a height of 18cm, a width of 8cm and a thi...

Embodiment 3

[0028] Example 3 The application of rhizosphere simulation culture device in researching the nutritional contribution of rhizosphere nutrients (taking glycine as an example)

[0029] The soils of Xianju, Xiaoshan, and Zijingang were collected and air-dried naturally to a water content of 15%. The soil was passed through a 3mm sieve and added to the pot described in Example 2. Two corn plants were planted. After 25 days of cultivation, the soil Is rhizosphere soil;

[0030] Add an abundance of 99.8% to the soil through a hollow tube to a concentration of 0.01mmolL -1 of 13 C, 15 30ml of N-Gly solution (add 5ml to each hollow tube), 4h after absorbing, cut the basin, take out the polyethylene hollow tube from the soil, collect root samples from the soil, and determine the root carbon and nitrogen abundance by isotope mass spectrometry , To study the effect of different soil texture on the nutritional contribution of rhizosphere molecular amino acids.

[0031] Table 2

[0032]

[0033] ...

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Abstract

The invention provides a rhizosphere simulated culture apparatus. The rhizosphere simulated culture apparatus is composed of a pot body, hollow pipes and polyethylene nets. The hollow pipes are inserted in the pot body in an inclined mode. An included angle ranging from 75 degrees to 85 degrees is formed between each hollow pipe and the pot body. The polyethylene nets tightly wrap the hollow pipes. The rhizosphere simulated culture apparatus is reasonable in design, easy to manufacture and low in cost, can effectively avoid the problem that root systems of square root boxes grow in a single direction, and solves the problems that the edges of root systems in columnar root boxes grow vigorously and root systems are lacking in soil bodies. By means of the rhizosphere simulated culture apparatus, nutrient substances can be evenly added to soil bodies in a directed mode by researching nutrition contribution of rhizosphere nutrient substances, and the problem that nutrient substances are not uniform when added from the upper portion of a culture box is avoided. The rhizosphere environment can be simulated, the nutrition contribution of rhizosphere nutrient substances and the interaction of root systems and soil can be researched. The problem that transverse growth of root systems in a square rhizosphere simulated culture apparatus is limited is effectively avoided, the state is closer to the natural growth state of root systems, and a method which is effective, convenient to use and accurate in result is provided for research of rhizosphere nutrient substance adsorption.

Description

Technical field [0001] The invention belongs to the field of soil science, and relates to a culture device and application, and in particular to a rhizosphere simulation culture device and application. Background technique [0002] The rhizosphere refers to the part of the micro-soil area that is different from the soil in physical, chemical and biological properties due to the influence of plant root activities on the soil. The rhizosphere is very small, generally within a few millimeters from the surface of the root shaft. Many chemical conditions and biochemical processes in the rhizosphere are different from the soil. The rhizosphere is the center of interaction between plants, soil, microorganisms and their environment, and is the area where the material and energy exchange between plants and the soil environment is most intense. The secretions released by plant roots strongly change the biological and chemical processes of the rhizosphere, which can not only activate soil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G9/02G01N33/24
CPCA01G9/02G01N33/24
Inventor 马庆旭吴良欢
Owner ZHEJIANG UNIV
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