Multieffect factor composition and preparation method and application thereof

A composition and factor technology, applied in the field of medical cosmetology, can solve the problems of short maintenance time, no basal cell damage repair function, single drug, etc., and achieve high activity, proliferation ability, good multi-directional differentiation potential, and high cell activity. Effect

Pending Publication Date: 2019-09-13
北京恒峰铭成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a pleiotropic factor composition for water phototherapy, aiming to solve the water treatment effect of single medicine, short maintenance time, simple filling or inflammation repair function without basal cell damage repair function technical issues

Method used

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  • Multieffect factor composition and preparation method and application thereof
  • Multieffect factor composition and preparation method and application thereof
  • Multieffect factor composition and preparation method and application thereof

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preparation example Construction

[0060] The embodiment of the present invention also provides a preparation method of the pleiotropic factor composition, comprising the following steps:

[0061] S10. Obtain the pleiotropic factor solution for hypoxic pretreatment;

[0062] S20. Obtain the water of formula quantity, sterilize, obtain sterilized water;

[0063] S30. Add the sodium hyaluronate in the prescribed amount to the sterilized water at a temperature of 60° C. to 75° C., stir and dissolve to obtain a sodium hyaluronate aqueous solution;

[0064] S40. Obtain formula quantities of mannitol, hypoxic pretreated pleiotropic factor solution, sodium ascorbate, panthenol, and phenoxyethanol, add them to the sodium hyaluronate aqueous solution cooled to 28°C-33°C, and mix well A pleiotropic factor composition is obtained.

[0065] In the preparation method of the pleiotropic factor composition provided in the embodiment of the present invention, after obtaining the hypoxic pretreated pleiotropic factor solution, ...

Embodiment 1

[0093] Preparation of pleiotropic factor solution for hypoxic preconditioning of umbilical cord mesenchymal stem cells.

[0094] Operate in an ultra-clean workbench, take a fresh full-term healthy fetal umbilical cord, wash it with normal saline containing 2 times 100U / mL penicillin and streptomycin to remove blood stains;

[0095]Peel off the Walton's jelly, cut the peeled Walton's jelly into blocks of less than 1 cubic millimeter, spread the block of Walton's jelly on the bottom of the cell culture device, and slowly pour it into the cell culture device. Add 10 mL of serum-free medium, and culture in a 37°C, 5% carbon dioxide, saturated humidity incubator.

[0096] After the primary cells were cultured for 7 days, the serum-free medium was replaced and cultured until the cells reached 70% confluence, the serum-free medium was removed, and trypsin-sodium edetate ( EDTA) digested for 1 min, the umbilical cord mesenchymal stem cells shrank and detached from the bottle wall;

...

Embodiment 2

[0102] Preparation of pleiotropic factor compositions.

[0103] Heat the water to a high temperature of 85°C, keep it warm for 20 minutes to sterilize, and obtain sterilized water;

[0104] Cool the above sterilized water to 60°C, add sodium hyaluronate, stir well until dissolved, and obtain a sodium hyaluronate aqueous solution;

[0105] Cool the above sodium hyaluronate aqueous solution to 30°C, add mannitol, sodium hyaluronate, mesenchymal stem cell hypoxic pretreated pleiotropic factor solution, sodium ascorbate, panthenol, and phenoxyethanol, and mix well. A pleiotropic factor composition is obtained.

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Abstract

The invention belongs to the technical field of medical cosmetology, and particularly relates to a multieffect factor composition, which includes: 0.01%-1% of hypoxic preconditioned multieffect factorsolution, 0.05%-1% of sodium hyaluronate, 2%-10% of mannitol, 0.5%-2% of sodium ascorbate, 0.5%-2% of panthenol and 0.05%-0.2% of PhenoXyaethanolum, with the banlance being water. The multieffect factor composition provided by the invention can carry out nourishing and tissue filling on skin, and cytokine clusters in the multieffect factor composition can also participate in the repair of basal cells in subcutaneous deep tissues, making the repair function of the multieffect factor composition provided by the embodiment of the invention more obvious.

Description

technical field [0001] The invention belongs to the technical field of medical cosmetology, and in particular relates to a pleiotropic factor composition and its preparation method and application. Background technique [0002] Aging has always been an eternal topic in the history of human existence, especially with age, the skin that the human body presents to the outside, including the skin of the face, neck, hands, etc., will also undergo tremendous changes with age , the most intuitive manifestation is that the skin is wrinkled and loose, the skin color becomes darker, and the texture spots increase. In addition, the influence of factors such as environmental factors, unhealthy diet and survival stress can also exacerbate the deterioration of skin conditions. At present, there are a variety of cosmetics and skin care products with anti-aging, anti-wrinkle, whitening and other effects on the market, but because the epidermis can only penetrate small molecular substances,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/73A61K8/34A61K8/67A61K8/42A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/34A61K8/345A61K8/42A61K8/676A61K8/735A61K8/99A61K2800/592A61Q19/00A61Q19/004A61Q19/02A61Q19/08
Inventor 郝好杰李梓源陈楠景浩然陈惠华周严恒
Owner 北京恒峰铭成生物科技有限公司
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