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Screening method and application of lactic acid bacteria strains with antioxidant capacity

A technology for fermenting bacteria and bacterial liquid concentration, which is applied in the biological field and can solve problems such as lactic acid bacteria that do not involve antioxidant properties

Active Publication Date: 2020-08-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents do not involve the application of lactic acid bacteria with antioxidant properties in fermented feed, nor do they study the changes in antioxidant levels of feed before and after microbial fermentation from the perspective of fermented feed

Method used

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  • Screening method and application of lactic acid bacteria strains with antioxidant capacity
  • Screening method and application of lactic acid bacteria strains with antioxidant capacity
  • Screening method and application of lactic acid bacteria strains with antioxidant capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: bacterial strain screening:

[0034] (1) Lactic acid bacteria isolated from traditional pickled vegetables

[0035] Take 1g of traditional pickled vegetables in Northeast China, dilute it with sterilized saline at a ratio of 1:10, bathe in 75°C water for 15 minutes, and then dilute it with sterile water 10 times gradient, select 10 -3 、10 -4 、10 -5 、10 -6 For four dilution gradients, draw 100 μL of the bacterial suspension and spread it on the MRS plate, at least 3 plates for each dilution gradient, culture at 37°C for about 48 hours to obtain a single colony, separate and purify by streaking, and obtain pure isolated lactic acid bacteria strains Several.

[0036] (2) Preliminary screening of lactic acid bacteria with hydrogen peroxide tolerance

[0037] Add 10ml of MRS medium into a stoppered test tube, and add an appropriate amount of hydrogen peroxide solution, so that the initial concentration of hydrogen peroxide in the medium is 0, 0.4, 0.7, 1.0...

Embodiment 2

[0063] Example 2: Physiological and biochemical characteristics and strain screening of Pediococcus pentosaceae ZJUAF-4

[0064] Table 4 Physiological and biochemical characteristics of bacterial strain ZJUAF-4 of the present invention

[0065]

[0066] Note: "+" means positive, "-" means negative

[0067] Identification of 16S rDNA of strain ZJUAF-4 of the present invention

[0068] The 16S rDNA of the bacterial strain ZJUAF-4 of the present invention was sequenced, and compared with the NCBI database by Blast, the results showed that the homology between ZJUAF-4 and the model strain Pediococcus pentosaceus NWAFU5032 reached 99%, and the homology with the model strain Pediococcus pentosaceus SD 7013 was 99%, and 99% homology with the model strain Pediococcuspentosaceus PEP1, so the strain was determined to be Pediococcus pentosaceus.

[0069] Strain ZJUFA-4 was deposited on November 21, 2018 at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, General Mic...

Embodiment 3

[0070] Embodiment 3: Application of Pediococcus pentosaceae ZJUAF-4 to prepare fermented feed

[0071] 1) Preparation of fermentation broth

[0072] Inoculate ZJUAF-4 in MRS liquid medium, and culture it statically for 24 hours, so that the concentration of bacteria solution reaches 1.0×10 9 ~1.0×10 10 CFU / mL, to prepare ZJUAF-4 fermentation broth;

[0073] 2) Preparation of fermented feed

[0074] Add 5% of the fermentation broth prepared in step 1) to the fermentation raw materials (including 10g of corn, 10g of soybean meal, 24g of bran, and 6g of soybean hulls), and then add and insert an appropriate amount of warm water so that the total moisture content accounts for 10% of the total mass of the fermentation system. 35% to 40%, stir and mix to obtain a fermentation system, leave it to ferment for 24 to 48 hours, and obtain a fermented feed.

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Abstract

The invention discloses a screening method and application of a lactic acid bacteria strain with antioxidant capacity. The Pediococcus pentosaceae ZJUFA-4 is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC NO: 16767. The strain was isolated from traditional pickled vegetables, and was obtained through preliminary screening of hydrogen peroxide tolerance and re-screening of DPPH free radical, hydroxyl free radical and superoxide anion free radical scavenging ability of the strain. The bacterial strain is used to ferment corn-soybean meal compound feed to increase the activity of antioxidant enzymes and the content of antioxidant substances in the fermented feed, so as to improve the growth performance and antioxidant level of fattening pigs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of development and application of functional lactic acid bacteria, in particular to a strain of lactic acid bacteria with antioxidant capacity and its application. Background technique [0002] Lactic acid bacteria is a general term for a class of bacteria that use fermentable carbohydrates to produce a large amount of lactic acid, have no spores, and are Gram-positive. They are widely distributed and diverse, including Lactobacillus, Lactococcus, Streptococcus, and Pediococcus. Facultative anaerobic microorganisms. Studies have shown that lactic acid bacteria have a variety of functions, not only can regulate the body's immune system, maintain the balance of intestinal flora, but also have a certain antioxidant capacity, by scavenging free radicals to keep the body in a relatively stable state, so it has antioxidant function Lactic acid bacteria can be used as a natural a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A23K10/12A23K10/18A23K50/30C12R1/01
CPCA23K10/12A23K10/18A23K50/30C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 汪以真郝丽红路则庆苏维发张煜王成王凤芹
Owner ZHEJIANG UNIV
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