Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tetrahydrocurcumin content detection method

A technology of tetrahydrocurcumin and detection method, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of no retention, increased polarity, etc., and achieves good repeatability and durability, simple operation, and good application prospects. Effect

Active Publication Date: 2019-09-20
SICHUAN ACAD OF CHINESE MEDICINE SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because Tetrahydrocurcumin is a weakly acidic compound, after the pH is increased, the hydroxyl group in its structure reacts with an alkali to form a salt (preparation route is as follows), the polarity increases, and there is almost no retention in reversed-phase chromatography (see figure 2 )

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tetrahydrocurcumin content detection method
  • Tetrahydrocurcumin content detection method
  • Tetrahydrocurcumin content detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Detection method of the present invention

[0036] 1) Preparation of reference substance solution: take 10 mg of tetrahydrocurcumin reference substance, accurately weigh it, put it in a 25ml measuring bottle, add methanol to the mark, ultrasonically oscillate to completely dissolve, shake well, and then accurately draw 1ml of it and place it in a 10ml measuring bottle , add methanol to the mark, shake well, and you get it;

[0037] 2) Preparation of the test solution: take 10 mg of tetrahydrocurcumin crude drug, accurately weighed, put in a 25 ml volumetric flask, add 20 ml of methanol, ultrasonically oscillate for 5 minutes, then dilute with methanol to the scale, shake well, and accurately measure the solution 1ml, diluted to 10ml, filtered, and the filtrate was used as the test solution;

[0038] 3) Take 10 μl of the reference substance solution and the test solution respectively, and inject them into an Agilent 1200 high-performance liquid chromatograp...

Embodiment 2

[0041] Embodiment 2 detection method of the present invention

[0042] 1) Preparation of reference substance solution: take 10 mg of tetrahydrocurcumin reference substance, accurately weigh it, add methanol to make a solution containing 0.05 mg of tetrahydrocurcumin per 1 ml, and obtain it;

[0043] 2) Preparation of the test solution: dissolve and quantitatively dilute the tetrahydrocurcumin raw material in methanol to prepare a solution containing 0.05 mg / ml of tetrahydrocurcumin as the test solution;

[0044] 3) Take 10 μl of the reference substance solution and the test solution respectively, and inject them into an Agilent 1200 high-performance liquid chromatograph (which includes a DAD detector), and the chromatographic conditions are as follows:

[0045] Chromatography column: Global Chromatography, GS-120-5-C18-BIO, 4.6×150mm, 5μm; detection wavelength 290nm; column temperature 25°C; flow rate 1.0ml / ml; mobile phase: acetonitrile-0.5% ammonia water (containing 0.01mol / ...

Embodiment 3

[0047] Embodiment 3 detection method of the present invention

[0048] 1) Preparation of reference substance solution: take 10 mg of tetrahydrocurcumin reference substance, accurately weigh it, add methanol to make a solution containing 0.05 mg of tetrahydrocurcumin per 1 ml, and obtain it;

[0049] 2) Preparation of the test solution: dissolve the contents of the tetrahydrocurcumin solid dispersion capsules with methanol, and quantitatively dilute to make a solution containing about 0.05 mg / ml of tetrahydrocurcumin, to obtain final product;

[0050]3) Take 10 μl of the reference substance solution and the test solution respectively, and inject them into an Agilent 1200 high-performance liquid chromatograph (which includes a DAD detector), and the chromatographic conditions are as follows:

[0051] Chromatographic column: Global Chromatography, GS-120-5-C18-BIO, 4.6×150mm, 5μm; detection wavelength 290nm; column temperature 35℃; flow rate 1.0ml / ml; mobile phase: acetonitrile-0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a tetrahydrocurcumin content detection method. The method comprises the following steps of 1) preparing a reference substance solution: taking tetrahydrocurcumin as a reference substance, dissolving and obtaining the reference substance solution; 2) preparing a test sample solution: taking a sample to be tested, dissolving and obtaining the test sample solution; and 3) injecting the reference substance solution and the test sample solution into a high performance liquid chromatograph, wherein chromatographic conditions are characterized in that a chromatographic column takes octadecyl silane bonded silica gel as a filling agent; a detection wavelength is within a range of 280 to 300nm; and a mobile phase is a mixed solution of an organic solvent and an alkaline aqueous solution. The method has simple operation, high precision, good stability, good repeatability and high accuracy. The method can be used to effectively evaluate quality of tetrahydrocurcumin bulk drugs.

Description

technical field [0001] The invention specifically relates to a method for detecting the content of tetrahydrocurcumin. Background technique [0002] Tetrahydrocurcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)heptane-3,5-dione) is a metabolite of curcumin in the body, which has antioxidant, anti-inflammatory, anti- The physiological effects of tumor, anti-diabetes, whitening and many other aspects are receiving more and more attention. Tetrahydrocurcumin is a compound containing β-diketone structure, which has two tautomers of ketone and enol, under the mobile phase conditions reported in the existing literature (acetonitrile / methanol-acid water system) , when using high performance liquid chromatography to measure tetrahydrocurcumin, the two isomers are separated on the chromatographic column, so that two peaks always appear in the chromatogram, and the shorter retention time is the ketone formula, which is shorter. The long one is the enol form (see figure 1 ). [0003] Due ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 朱宁凌海燕杨安东
Owner SICHUAN ACAD OF CHINESE MEDICINE SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com