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Primer design for retrieval of stored polynucleotides

A technology of polynucleotides and nucleotides, applied in the field of primer design for the retrieval of stored polynucleotides, capable of solving problems such as double-stranded polynucleotide sequence errors

Pending Publication Date: 2019-09-20
MICROSOFT TECH LICENSING LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, duplication of the binding site for the primer at a position other than the end of the nucleotide sequence may result in errors in the sequence of the double-stranded polynucleotide produced during the duplication

Method used

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  • Primer design for retrieval of stored polynucleotides
  • Primer design for retrieval of stored polynucleotides
  • Primer design for retrieval of stored polynucleotides

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Embodiment Construction

[0016] This disclosure describes techniques to increase the accuracy of random access to data encoded by polynucleotides and to increase the efficiency and specificity of replication and amplification of polynucleotide sequences in polynucleotide data storage systems. Most data generated by computing devices is stored on conventional data storage systems including various magnetic, optical, and / or solid-state storage media. The capacity of traditional data storage systems has not kept up with the rate at which computing devices are generating data. Polynucleotide sequences such as DNA can be used to store very large amounts of data on a scale that exceeds the capacity of traditional storage systems. An arrangement of nucleotides (eg, CTGAAGT...) included in a polynucleotide sequence may correspond to an arrangement of bits (eg, 11010001...) that encodes data. Data may include audio data, video data, image data, text data, software, combinations thereof, and the like.

[0017...

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Abstract

This disclosure describes techniques to improve the accuracy of random access of data stored in polynucleotide sequence data storage systems. Primers used in polynucleotide sequence replication and amplification can be scored against a number of criteria that indicate the fitness of sequences of nucleotides to function as primers. Primers having scores that indicate a particular fitness to function as primers can be added to a specific group of primers. The primers from the group of primers can be used in amplification and replication of polynucleotide sequences that encode digital data. Additionally, an amount of overlap between primer targets and payloads encoding digital data can be determined. Minimizing the amount of overlap between primer targets and payloads can improve the efficiency of polynucleotide replication and amplification. The bits of the digital data can be randomized to minimize the amount of overlap between payloads encoding the digital data and primer targets.

Description

Background technique [0001] A polynucleotide sequence can be arranged as a linear chain of organic molecules, which are nitrogenous bases such as adenine (A), guanine (G), thymine (T) in the case of deoxyribonucleic acid (DNA) , cytosine (C) and in the case of ribonucleic acid (RNA) T, G, C and uracil (U). Polynucleotide sequences can be naturally occurring or synthetic. In some cases, individual bases included in a polynucleotide sequence can pair with a complementary base in another polynucleotide sequence to create a double-stranded arrangement of polynucleotide sequences. For example, in the case of deoxyribonucleic acid (DNA), T and A are complementary, and G and C are complementary. In the case of ribonucleic acid (RNA), T and U are complementary, and G and C are complementary. [0002] Complementary nucleotides in two polynucleotide sequences can align with each other to form a double-stranded polynucleotide. The two ends (referred to as the 5' and 3' ends) of a pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/00G16B25/20
CPCG16B25/00G16B25/20G16B50/30C12Q1/6811
Inventor 陈圆觉K·施特劳斯L·H·塞兹S·D·安格S·耶卡尼恩
Owner MICROSOFT TECH LICENSING LLC