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A kind of preparation method of silk fibroin thixotropic gel

A technology of silk fibroin and thixotropy, which is applied in prosthetics, medical science, etc., can solve the problems of not being able to load protein active molecules and the impact of biocompatibility, and achieve increased work efficiency, no pollution to the environment, and simple follow-up operations Effect

Active Publication Date: 2022-02-11
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the use of organic alcohols will have a negative impact on the biocompatibility of the material, and it cannot be loaded with protein active molecules.

Method used

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  • A kind of preparation method of silk fibroin thixotropic gel
  • A kind of preparation method of silk fibroin thixotropic gel
  • A kind of preparation method of silk fibroin thixotropic gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare silk fibroin solution. Specifically: put 60g silk in 0.02M Na 2 CO 3 Boil in the solution at 100°C for 30 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat the above operation 3 times, and then dry the silk at 60°C. 30 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of LiBr solution with a concentration of 9.3 mol / L, and dissolved at 60° C. for 4 hours. Then use a dialysis bag with a molecular weight cut-off of 3500 to soak in deionized water for dialysis for 3 days, during which the water is changed every two hours to remove LiBr in the solution, thereby obtaining a pure silk fibroin solution.

[0028] The pH of the silk fibroin solution was adjusted to around 4.0 with dilute hydrochloric acid, diluted to 1% with deionized water, placed in an oven for 24 hours, and then ultrasonicated for 30 seconds with an ultrasonic cell disruptor to obtain a crystalline silk fibroin solution.

[0...

Embodiment 2

[0032] The pure silk fibroin solution was prepared by the same method as in Example 1.

[0033] After the silk fibroin solution was concentrated at 60° C. for 48 hours, it was diluted to 1% with deionized water, placed in an oven for 24 hours, and then ultrasonicated for 30 seconds with an ultrasonic cell disruptor to obtain a crystalline silk fibroin solution.

[0034] Dilute the ordinary silk fibroin solution to 1%, and then mix it with the above silk fibroin nanofiber solution at a volume ratio of 1:10 at room temperature, and obtain a silk fibroin thixotropic gel after 1 hour.

[0035] See figure 2 , figure 2 It is an optical image of the silk fibroin thixotropic gel described in Example 1. From figure 2 It can be seen that the gel has a very high transparency.

Embodiment 3

[0037] The pure silk fibroin solution and the silk fibroin nanofiber solution were prepared by the same method as in Example 2.

[0038] Dilute the ordinary silk fibroin solution to 1.5%, then mix it with the above silk fibroin nanofiber solution at a volume ratio of 90:1, let it stand at 0°C, and obtain a silk fibroin thixotropic gel after 96 hours .

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Abstract

The invention discloses a preparation method of silk fibroin thixotropic gel. Firstly, common silk fibroin solution and crystallized silk fibroin particle solution are prepared, then the crystallized silk fibroin particle solution is mixed with common silk fibroin solution of specific concentration, and left standing to obtain silk fibroin thixotropic gel. This technology can control the gelation time and light transmittance of hydrogel by changing the concentration of silk fibroin solution, the concentration of crystallized silk fibroin particle solution, the mixing ratio of the two, and the treatment temperature. The preparation process of the present invention is mild, no toxic organic reagents such as cross-linking agents are needed, the preparation conditions are green and controllable, it is convenient for large-scale preparation, there is no waste residue, green and pollution-free, and it is easy for industrialization.

Description

technical field [0001] The invention relates to a thixotropic gel and a preparation method thereof, in particular to a preparation technology for reversible and recyclable gel which uses silk fibroin as a raw material and does not require organic solvents or other additives. Background technique [0002] Tissue engineering is a potential way to solve the clinical organ defect problem. Tissue engineering is to inoculate and cultivate cells on artificially constructed scaffold materials to regenerate tissues or organs under the action of nutrients such as growth factors, and to repair or replace the function of damaged tissues or organs. Choosing the right scaffold material is of great significance to tissue engineering technology. [0003] As a natural protein material, silk fibroin is not only easy to purify and process, but also has good biocompatibility, biodegradable speed and adjustable physical and chemical properties, which makes this material as a tissue repair scaff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L89/00A61L27/22A61L27/52A61L27/50
CPCC08J3/075A61L27/227A61L27/52A61L27/50C08J2389/00C08J2489/00C08L89/00
Inventor 姚丹语史廷春
Owner HANGZHOU DIANZI UNIV
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