Polypeptide for preparing echinococcosis granulosa diagnostic reagent and application thereof

Abstract

The invention discloses a polypeptide for preparing an echinococcosis granulosa diagnostic reagent and application thereof. The polypeptide for preparing the echinococcosis granulosa diagnostic reagent is eB2-3 and / or eB3-3, eB2-3 is the polypeptide with the amino acid sequence shown in sequence 1 in a sequence table, and eB3-3 is the polypeptide with the amino acid sequence shown in sequence 2 inthe sequence table An antibody detection kit prepared by taking an eB2-3 conjugate and / or eB3-3 conjugate obtained by coupling the polypeptide and carrier protein as a coating antigen is high in sensibility, high in accuracy and easy and rapid to operate, and the kit is applicable to mass screening detection of grass-roots veterinary departments at all levels and Entry-Exit Inspection and Quarantine Bureau on echinococcus granulosus infection serum antibodies.

Description

technical field [0001] The invention relates to polypeptides for preparing diagnostic reagents for echinococcosis granulosus and applications thereof. Background technique [0002] Echinococcus granulosus is also known as Echinococcus granulosus. Adults parasitize canine carnivores, while larvae (echinococcus) mainly parasitize herbivores such as humans, cattle, and sheep, causing a serious zoonotic disease called echinococcosis granulosus. When sheep eat the ovum proglottis or eggs of Echinococcus granulosus excreted by dogs, the hexacarpal larvae in the eggs escape from the digestive tract, penetrate into the intestinal wall, and spread to all parts of the body along with blood and lymph Develop into adults, causing echinococcosis. There is generally no good treatment for sheep suffering from echinococcosis. After the infection is found, the culling and the harmless treatment of the corpse are mainly to prevent the viscera from being eaten by dogs, causing adult worms to...

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Problems solved by technology

[0003] Jiang Li et al. used the pET32a vector, pET28a vector and pGEX4T-1 vector to express the AgB1 and AgB2 subunit genes cloned from the isolates of Echinococcus granulosus (Xinjiang source and Gansu source) in China. The results showed that AgB1 could not be expressed in the pET28a vector. Expressed, but can be expressed in pGEX4T-1 and pET32a, but the expressed proteins are all insoluble

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