Magnetic nano polystyrene carboxyl microspheres and preparation method thereof

Abstract

The invention relates to magnetic nano polystyrene carboxyl microspheres and a preparation method thereof. The magnetic nano polystyrene carboxyl microspheres are obtained by firstly synthesizing crosslinked polystyrene carboxyl microspheres with stable carboxy groups on the surface by a polymerization reaction of a styrene monomer, a crosslinking agent and gadoleic acid, removing large particlesin the microspheres, maintaining a uniform particle size of 60 nm to 300 nm, then dispersing uniform crosslinked nano polystyrene carboxyl microspheres into an iron / cobalt / manganese / nickel salt solution containing a reductive organic solvent to form homogeneous liquid, performing a high-pressure thermal insulation reaction on the homogeneous liquid under high temperature and high pressure conditions to synthesize composite magnetic nanoparticles, and removing the magnetic particles on the surface of the composite magnetic nanoparticles with acid. The magnetic nano polystyrene carboxyl microspheres prepared by the method involved in the invention have the advantages of good magnetic responsiveness, uniform particle size, good monodispersity, uniform magnetic content of particles, good magnetic particle embedding effect, stable functional group modification, and the like.

Description

technical field

[0001] The invention relates to the technical field of composite material preparation, in particular to a magnetic nano polystyrene carboxyl microsphere and a preparation method thereof. Background technique

[0002] Magnetic beads are composite functional microspheres with superparamagnetism. Because of their surface modification with functional groups such as amino groups and carboxyl groups, they can be coupled to proteins, DNA, etc. Therefore, it has a wide range of applications in cell separation, immobilized enzymes, immunodiagnosis, tumor targeted therapy, and DNA extraction. In actual operation, well-applied nano magnetic beads must have uniform particle size, consistent magnetic content of each particle, good magnetic particle embedding effect, good monodispersity, stable functional group modification and good magnetic response. The current common preparation method of nano magnetic beads is to 3 o 4 Surface modified polymer layer, on the one hand...