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Application of siRNA used for inhibiting NPC1 gene expression

A gene expression and reaction technology, applied in DNA/RNA fragments, genetic engineering, recombinant DNA technology, etc., can solve the problem of limited drug resistance of 5-fluorouracil, and achieve the effect of reducing drug resistance

Active Publication Date: 2019-10-01
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]However, 5-fluorouracil has limited efficacy and there is a need to develop new drugs for the treatment of colorectal cancer

Method used

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  • Application of siRNA used for inhibiting NPC1 gene expression
  • Application of siRNA used for inhibiting NPC1 gene expression
  • Application of siRNA used for inhibiting NPC1 gene expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment provides a drug for treating colorectal cancer, which includes NPC1-siRNA-1 and 5-fluorouracil, the nucleotide sequence of the sense strand of NPC1-siRNA-1 is shown in SEQ ID NO.1, and its reaction chain The nucleotide sequence is shown in SEQ ID NO.2.

Embodiment 2

[0040]This embodiment provides a drug for treating colorectal cancer, which includes NPC1-siRNA-3 and 5-fluorouracil, the nucleotide sequence of the sense strand of NPC1-siRNA-1 is shown in SEQ ID NO.3, and its reaction chain The nucleotide sequence is shown in SEQ ID NO.4.

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Abstract

The invention discloses application of siRNA used for inhibiting NPC1 gene expression, and belongs to the technical field of biological medicine. The siRNA used for inhibiting the NPC1 gene expressionis at least one of the following two siRNAs of NPC1-siRNA-1 and NPC1-siRNA-3; for the NPC1-siRNA-1, the nucleotide sequence of a positive-sense strand is as shown in SEQ ID NO.1, and the nucleotide sequence of a reaction chain is as shown in SEQ ID NO.2; for the NPC1-siRNA-3, the nucleotide sequence of a positive-sense strand is as shown in SEQ ID NO.3, and the nucleotide sequence of a reaction chain is as shown in SEQ ID NO.4. Accordingly, the designed NPC1-siRNA-1 and NPC1-siRNA-3 can well inhibit the NPC1 gene expression in HCT-116 and HCT-8 cells respectively; under NPC1-siRNA-1 and NPC1-siRNA-3 transfection, the drug resistance effect of tumor cells knocking down NPC1 on 5-FU is lowered; the designed NPC1-siRNA and 5-fluorouracil can be combined to be used for treatment of the colorectal cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of siRNA for inhibiting NPC1 gene expression. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive tract, accounting for the second place among gastrointestinal tumors. The most common site of colorectal cancer is the junction of the rectum and the sigmoid colon, accounting for 60%; the incidence is mostly after the age of 40. 5-Fluorouracil can be converted into different cytotoxic metabolites in cells, and acts on S phase, and belongs to anti-metabolism anti-tumor drugs. [0003] However, 5-fluorouracil has limited medicinal properties, and it is necessary to develop new drugs for the treatment of colorectal cancer. Contents of the invention [0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide the application of siRNA inhibiting NPC1 gene expression,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61K31/513A61P35/00
CPCC12N15/113A61K31/513A61K31/713A61P35/00C12N2310/14A61K2300/00
Inventor 叶树标兰平杨孜欢陈代词李佩思冯杏芝
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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